Insulinotropin PACAP potentiates insulin-stimulated glucose uptake in 3T3 L1 cells

被引：28

作者：

Nakata, M

Shioda, S

Oka, Y

Maruyama, I

Yada, T

机构：

[1] Kagoshima Univ, Fac Med, Dept Physiol, Kagoshima 8908520, Japan

[2] Showa Univ, Sch Med, Dept Anat, Tokyo 1428555, Japan

[3] Yamaguchi Univ, Sch Med, Dept Internal Med 3, Ube, Yamaguchi 7558505, Japan

[4] Natl Inst Physiol Sci, Lab Intracellular Metab, Okazaki, Aichi 4448585, Japan

来源：

PEPTIDES | 1999年 / 20卷 / 08期

关键词：

PACAP; PAC(1) receptor; insulin; adipocyte; 3T3-L1; glucose uptake; PI; 3-kinase; tyrosine-phosphorylation;

D O I：

10.1016/S0196-9781(99)00085-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pituitary adenylate cyclase-activating polypeptide (PACAP) is localized in pancreatic nerve fibers and islets and potently augments glucose-induced insulin secretion. The present study explored a possible extra-pancreatic action of PACAP. The specific PACAP receptor (PAC, receptor) was expressed in the rat fat tissue and 3T3-L1 adipocytes. PACAP-38 (10 nM) significantly enhanced insulin-induced 2-deoxyglucose uptake by 3T3-L1 adipocytes. Insulin-stimulated phosphatidylinositol 3-kinase activity was further increased by PACAP-38, whereas the tyrosine-phosphorylation of insulin receptor beta-subunit and insulin receptor substrate-1 was unaltered by PACAP-38. These results reveal that PACAP-38 enhances insulin-induced glucose uptake. an effect probably mediated by insulin-stimulated phosphatidylinositol 3-kinase, and that PACAP potentiates not only insulin secretion, but also insulin action in adipocytes. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：943 / 948

页数：6

共 28 条

[1] Signaling mechanisms underlying the insulinotropic effect of pituitary adenylate cyclase-activating polypeptide in HIT-T15 cells [J].