Development and Characterization of Microfluidic Devices and Systems for Magnetic Bead-Based Biochemical Detection

被引：80

作者：

Choi, Jin-Woo ^{[1
,2
]}

Oh, Kwang W. ^{[1
,2
]}

Han, Arum ^{[1
,2
]}

Okulan, Nihat ^{[1
,2
]}

Wijayawardhana, C. Ajith ^{[3
]}

Lannes, Chad ^{[1
,2
]}

Bhansali, Shekhar ^{[1
,2
]}

Schlueter, Kevin T. ^{[3
]}

Heineman, William R. ^{[3
]}

Halsall, H. Brain ^{[3
]}

Nevin, Joseph H. ^{[1
,2
]}

Helmicki, Arthur J. ^{[1
,2
]}

Henderson, H. Thurman ^{[1
,2
]}

Ahn, Chong H. ^{[1
,2
]}

机构：

[1] Univ Cincinnati, Ctr Microelect Sensors, Cincinnati, OH 95221 USA

[2] Univ Cincinnati, MEMS, Dept Elect & Comp Engn & Comp Sci, Cincinnati, OH 95221 USA

[3] Univ Cincinnati, Dept Chem, Cincinnati, OH 45221 USA

来源：

BIOMEDICAL MICRODEVICES | 2001年 / 3卷 / 03期

关键词：

microfluidic system; magnetic bead; immunoassay; biochemical detection;

D O I：

10.1023/A:1011490627871

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This paper presents the development and characterization of a generic microfluidic system for magnetic bead-based biochemical detection. Microfluidic and electrochemical detection devices such as microvalves, flow sensors, biofilters, and immunosensors have been successfully developed and individually characterized in this work. Magnetically driven microvalves, pulsed-mode microflow sensors, magnetic particle separators as biofilters, and electrochemical immunosensors have been separately fabricated and tested. The fabricated microfluidic components have been surface-mounted on the microfluidic motherboard for fully integrated microfluidic biochemical detection system. A magnetic bio-bead approach has been adopted for both sampling and manipulating target biological molecules. Magnetic beads were used as both substrate of antibodies and carriers of target antigens for magnetic bead-based immunoassay, which was chosen as a proof-of-concept for the generic microfluidic biochemical detection system. The microfluidic and electrochemical immunosensing experiment results obtained from this work have shown that the biochemical sensing capability of the complete microfluidic subsystem is suitable for portable biochemical detection of bio-molecules. The methodology and system, which has been developed in this work, can be extended to generic bio-molecule detection and analysis systems by replacing antibody/antigen with appropriate bio receptors/reagents such as DNA fragments or oligonucleotides for application towards DNA analysis and/or high throughput protein analysis.

引用

页码：191 / 200

页数：10

共 15 条

[1] AHN CH, 1998, P MICR TOT AN SYST 9, P225
[2] BISSON C, 1998, SOL STAT SENS ACT WO, P1
[3] Björefors F, 2000, ELECTROANAL, V12, P255
[4] A new magnetic bead-based, filterless bio-separator with planar electromagnet surfaces for integrated bio-detection systems
Choi, JW
Ahn, CH
Bhansali, S
Henderson, HT
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2000, 68 (1-3) : 34 - 39
[5] Han A., 2000, Proceedings IEEE Thirteenth Annual International Conference on Micro Electro Mechanical Systems (Cat. No.00CH36308), P414, DOI 10.1109/MEMSYS.2000.838553
[6] Harrison DJ, 1996, SENSOR ACTUAT B-CHEM, V33, P105, DOI 10.1016/0925-4005(96)01932-6
[7] HEINEMAN WR, 1999, PITTCON 99 ORL FL MA
[8] Integrated system for rapid PCR-based DNA analysis in microfluidic devices
Khandurina, J
McKnight, TE
Jacobson, SC
Waters, LC
Foote, RS
Ramsey, JM
[J]. ANALYTICAL CHEMISTRY, 2000, 72 (13) : 2995 - 3000
[9] SMALL-VOLUME VOLTAMMETRIC DETECTION OF 4-AMINOPHENOL WITH INTERDIGITATED ARRAY ELECTRODES AND ITS APPLICATION TO ELECTROCHEMICAL ENZYME-IMMUNOASSAY
NIWA, O
XU, Y
HALSALL, HB
HEINEMAN, WR
[J]. ANALYTICAL CHEMISTRY, 1993, 65 (11) : 1559 - 1563
[10] Okulan N., 1998, SOL STAT SENS ACT WO, P320

← 1 2 →