Calcium, calmodulin, and CaMKII requirement for initiation of centrosome duplication in Xenopus egg extracts

被引：81

作者：

Matsumoto, Y

Maller, JL ^{[1
]}

机构：

[1] Univ Colorado, Sch Med, Howard Hughes Med Inst, Denver, CO 80262 USA

[2] Univ Colorado, Sch Med, Dept Pharmacol, Denver, CO 80262 USA

来源：

SCIENCE | 2002年 / 295卷 / 5554期

关键词：

D O I：

10.1126/science.1065693

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aberrant centrosome duplication is observed in many tumor cells and may contribute to genomic instability through the formation of multipolar mitotic spindles. Cyclin-dependent kinase 2 (Cdk2) is required for multiple rounds of centrosome duplication in Xenopus egg extracts but not for the initial round of replication. Egg extracts undergo periodic oscillations in the level of free calcium. We show here that chelation of calcium in egg extracts or specific inactivation of calcium/calmodulin-dependent protein kinase 11 (CaMKII) blocks even initial centrosome duplication, whereas inactivation of Cdk2 does not. Duplication can be restored to inhibited extracts by addition of CaMKII and calmodulin. These results indicate that calcium, calmodulin, and CaMKII are required for an essential step in initiation of centrosome duplication. Our data suggest that calcium oscillations in the cell cycle may be linked to centrosome duplication.

引用

页码：499 / 502

页数：4

共 27 条

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