Isolation and spectral characterization of Photosystem II reaction center from Synechocystis sp PCC 6803

被引：17

作者：

Tomo, Tatsuya ^{[1
]}

Akimoto, Seiji ^{[2
,3
]}

Tsuchiya, Tohru ^{[1
]}

Fukuya, Michitaka ^{[3
]}

Tanaka, Kazunori ^{[3
]}

Mimuro, Mamoru ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Human & Environm Studies, Sakyo Ku, Kyoto 6068501, Japan

[2] Kobe Univ, Mol Photosci Res Ctr, Kobe, Hyogo 6578501, Japan

[3] Kobe Univ, Grad Sch Sci, Kobe, Hyogo 6578501, Japan

来源：

PHOTOSYNTHESIS RESEARCH | 2008年 / 98卷 / 1-3期

关键词：

Photosystem II; Reaction center; Cyanobacteria; Chlorophyll; Pheophytin; beta-Carotene;

D O I：

10.1007/s11120-008-9354-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We isolated highly-purified photochemically active photosystem (PS) II reaction center (RC) complexes from the cyanobacterium Synechocystis sp. PCC 6803 using a histidine-tag introduced to the 47 kDa chlorophyll protein, and characterized their spectroscopic properties. Purification was carried out in a one-step procedure after isolation of PS II core complex. The RC complexes consist of five polypeptides, the same as in spinach. The pigment contents per two molecules of pheophytin a were 5.8 +/- 0.3 chlorophyll (Chl) a and 1.8 +/- 0.1 beta-carotene; one cytochrome b(559) was found per 6.0 Chl a molecules. Overall absorption and fluorescence properties were very similar to those of spinach PS II RCs; our preparation retains the best properties so far isolated from cyanobacteria. However, a clear band-shift of pheophytin a and beta-carotene was observed. Reasons for these differences, and RC composition, are discussed on the basis of the three-dimensional structure of complexes.

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页码：293 / 302

页数：10

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