BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells

被引：108

作者：

Bai, Yan ^{[1
]}

Li, Peipei ^{[1
]}

Yin, Guangfu ^{[1
]}

Huang, Zhongbing ^{[1
]}

Liao, Xiaoming ^{[1
]}

Chen, Xianchun ^{[1
]}

Yao, Yadong ^{[1
]}

机构：

[1] Sichuan Univ, Coll Mat Sci & Engn, Chengdu 610064, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 03期

基金：

中国国家自然科学基金;

关键词：

BMP-2; bFGF; MSC; Osteogenic differentiation; VEGF; ENDOTHELIAL GROWTH-FACTOR; OSTEOBLASTIC DIFFERENTIATION; REGENERATION; ENHANCEMENT; MODULATION; DELIVERY;

D O I：

10.1007/s10529-012-1084-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Mesenchymal stem cells (MSCs) were treated with bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) dose-dependently and time-dependently. Together they caused a strong synergistic effect on the osteogenic differentiation of MSCs, with lower concentrations of each factor being enough to show the synergistic promotion (50 ng BMP-2/ml, 1 ng VEGF/ml and 10 ng bFGF/ml). When both VEGF and bFGF were added in the early proliferating stage (the first 7 days) and BMP-2 was added in the late differentiation stage (the last 7 days), osteogenic differentiation of MSCs could be enhanced more effectively.

引用

页码：301 / 308

页数：8

共 20 条

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Vascular endothelial growth factor can signal through platelet-derived growth factor receptors [J].