Application of nonaqueous capillary electrophoresis for quantitative analysis of quinolizidine alkaloids in Chinese herbs

被引：61

作者：

Li, YQ ^{[1
]}

Cui, SY ^{[1
]}

Cheng, YQ ^{[1
]}

Chen, XG ^{[1
]}

Hu, ZD ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 508卷 / 01期

基金：

中国国家自然科学基金;

关键词：

fangchinoline; tetrandrine; sinomenine; tetrahydropalmatine; nonaqueous capillary electrophoresis; Chinese herbs;

D O I：

10.1016/j.aca.2003.11.043

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An easy, rapid method for simultaneous determination of tetrandrine (TET), fangchinoline (FAN), sinomenine (SIN) and tetrahydropalmatine (TEP) in Chinese herbs was developed by nonaqueous capillary electrophoresis without pretreatment for the first time. Optimum separation was achieved with a fused-silica capillary column (50 cm x 75 mu i.d.) and a running buffer containing 50 mM ammonium acetate, 1.0% acetic acid and 20% acetonitrile in methanol medium. The applied voltage was 20.0 kW The analytes were detected by UV at 214 nm. The effects of concentration of ammonium acetate, acetic acid and organic modifier on electrophoretic behaviour of the analytes were studied. Regression equations revealed linear relationships (correlation coefficients: 0.9991-0.9999) between the peak area of each analyte and the concentration. The levels of analytes in Stephania tetrandra S. Moore, Rhizoma corydalis and Sinomenium acutum Rehd. et. Wils were easily determined with recoveries ranging from 96 to 107%. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：17 / 22

页数：6

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