Identification of sigma(S)-regulated genes in Salmonella typhimurium: Complementary regulatory interactions between sigma(S) and cyclic AMP receptor protein

被引:36
作者
Fang, FC
Chen, CY
Guiney, DG
Xu, YS
机构
[1] UNIV COLORADO,HLTH SCI CTR,DEPT PATHOL,DENVER,CO 80262
[2] UNIV COLORADO,HLTH SCI CTR,DEPT MICROBIOL,DENVER,CO 80262
[3] UNIV CALIF SAN DIEGO,DEPT MED,LA JOLLA,CA 92093
关键词
D O I
10.1128/jb.178.17.5112-5120.1996
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
sigma(s) (RpoS)-regulated lacZ transcriptional fusions in Salmonella typhimurium were identified from a MudJ transposon library by placing the rpoS gene under the control of the araBAD promoter and detecting lacZ expression in the presence or absence of arabinose supplementation. Western blot (immunoblot) analysis of bacteria carrying P-BAD::rpoS demonstrated arabinose-dependent rpoS expression during all phases of growth. sigma(s)-dependent gene expression of individual gene fusions was confirmed by P22-mediated transduction of the MudJ insertions into wild-type or rpoS backgrounds. Analysis of six insertions revealed the known sigma(s)-regulated gene otsA, as well as five novel loci. Each of these genes is maximally expressed in stationary phase, and all but one show evidence of cyclic AMP receptor protein-dependent repression during logarithmic growth which is relieved in stationary phase. For these genes, as well as for the sigma(s)-regulated spvB plasmid virulence gene, a combination of rpoS overexpression and crp inactivation can result in high-level expression during logarithmic growth. The approach used to identify sigma(s)-regulated genes in this study provides a general method for the identification of genes controlled by trans-acting regulatory factors.
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收藏
页码:5112 / 5120
页数:9
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