High-resolution secondary ion mass spectrometry analysis of carbon dynamics in mycorrhizas formed by an obligately myco-heterotrophic orchid

被引:41
作者
Bougoure, Jeremy [1 ]
Ludwig, Martha [2 ]
Brundrett, Mark [4 ]
Cliff, John [3 ]
Clode, Peta [3 ]
Kilburn, Matt [3 ]
Grierson, Pauline [2 ]
机构
[1] Univ Western Australia, Sch Earth & Environm, Crawley, WA 6009, Australia
[2] Univ Western Australia, Sch Plant Biol, Crawley, WA 6009, Australia
[3] Univ Western Australia, Ctr Microscopy Characterisat & Anal, Crawley, WA 6009, Australia
[4] Dept Environm & Conservat, Bentley, WA 6983, Australia
基金
澳大利亚研究理事会;
关键词
achlorophyllous orchid; nanoSIP; nanoSIMS; Rhizanthella gardneri; mycorrhiza; UNDERGROUND ORCHID; GREEN; NITROGEN;
D O I
10.1111/pce.12230
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Mycorrhiza formation represents a significant carbon (C) acquisition alternative for orchid species, particularly those that remain achlorophyllous through all life stages. As it is known that orchid mycorrhizas facilitate nutrient transfer (most notably of C), it has not been resolved if C transfer occurs only after lysis of mycorrhizal structures (fungal pelotons) or also across the mycorrhizal interface of pre-lysed pelotons. We used high-resolution secondary ion mass spectrometry (nanoSIMS) and labelling with enriched (CO2)-C-13 to trace C transfers, at subcellular scale, across mycorrhizal interfaces formed by Rhizanthella gardneri, an achlorphyllous orchid. Carbon was successfully traced in to the fungal portion of orchid mycorrhizas. However, we did not detect C movement across intact mycorrhizal interfaces up to 216 h post (CO2)-C-13 labelling. Our findings provide support for the hypothesis that C transfer from the mycorrhizal fungus to orchid, at least for R. gardneri, likely occurs after lysis of the fungal peloton.
引用
收藏
页码:1223 / 1230
页数:8
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