Chitosan-coated PLGA nanoparticles: A sustained drug release strategy for cell cultures

被引：136

作者：

Chronopoulou, Laura ^{[1
]}

Massimi, Mara ^{[2
]}

Giardi, Maria Federica ^{[2
]}

Cametti, Cesare ^{[3
]}

Devirgiliis, Laura Conti ^{[4
]}

Dentini, Mariella ^{[1
]}

Palocci, Cleofe ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

[2] Univ Aquila, Dept Life Hlth & Environm Sci, I-67100 Laquila, Italy

[3] Univ Roma La Sapienza, Dept Phys, I-00185 Rome, Italy

[4] Univ Roma La Sapienza, Dept Biol & Biotechnol Charles Darwin, I-00185 Rome, Italy

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2013年 / 103卷

关键词：

Drug delivery; Nanoparticle uptake; Biopolymers; POLYMERIC NANOPARTICLES; IN-VIVO; DELIVERY; ACID; SYSTEM;

D O I：

10.1016/j.colsurfb.2012.10.063

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

A recently patented one-step methodology was used for the formulation of chitosan (CS) coated polylactic-co-glycolic acid (PLGA) nanoparticles containing dexamethasone (DXM) as a model drug. SEM investigations showed that nanoparticles (NPs) were spherical in shape with smooth surface. CS coating switched NPs zeta-potential from negative to positive, without modifying particle size distribution. Moreover, CS coating allowed a significant modulation of in vitro drug release, providing a sustained drug delivery in cultured cells. The uptake of fluorescent CS-coated PLGA NPs by hepatocytes (C3A) and fibroblasts (3T6) as well as the fate of internalized NPs were investigated by confocal microscopy. 3T6 and C3A cells were treated with DXM-loaded NPs and experiments were addressed to analyze the specific cell response to DXM, in order to evaluate its functional efficiency in comparison with conventional addition to culture medium. CS-coating of DXM loaded PLGA NPs allowed their uptake by cultured cells without inducing cytotoxicity. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：310 / 317

页数：8

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