Guidelines for authors and reviewers on antibody use in physiology studies

被引：70

作者：

Brooks, Heddwen L. ^{[1
]}

Lindsey, Merry L. ^{[2
,3
]}

机构：

[1] Univ Arizona, Coll Med, Dept Physiol Pharmacol & Med, Sarver Heart Ctr, Tucson, AZ USA

[2] Univ Mississippi, Med Ctr, Dept Physiol & Biophys, Mississippi Ctr Heart Res, Jackson, MS 39216 USA

[3] GV Sonny Montgomery Vet Affairs Med Ctr, Res Serv, Jackson, MS USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2018年 / 314卷 / 04期

基金：

美国国家卫生研究院;

关键词：

antibody; immunoblot analysis; immunoblotting; immunohistochemistry; rigor and reproducibility; OVERLOAD HEART-FAILURE; MYOCARDIAL-INFARCTION; POSTMYOCARDIAL INFARCTION; CARDIAC-HYPERTROPHY; ANGIOTENSIN-II; PPAR-ALPHA; PROTEIN; ACTIVATION; VALIDATION; MICE;

D O I：

10.1152/ajpheart.00512.2017

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Antibody use is a critical component of cardiovascular physiology research, and antibodies are used to monitor protein abundance (immunoblot analysis) and protein expression and localization (in tissue by immunohistochemistry and in cells by immunocytochemistry). With ongoing discussions on how to improve reproducibility and rigor, the goal of this review is to provide best practice guidelines regarding how to optimize antibody use for increased rigor and reproducibility in both immunoblot analysis and immunohistochemistry approaches. Listen to this article's corresponding podcast at http://ajpheart.podbean.com/e/guidelines-on-antibody-use-in-physiology-studies/.

引用

收藏

页码：H724 / H732

页数：9

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