Cellular response to phase-separated blends of tyrosine-derived polycarbonates

被引:16
作者
Bailey, LO
Becker, ML [1 ]
Stephens, JS
Gallant, ND
Mahoney, CM
Washburn, NR
Rege, A
Kohn, J
Amis, EJ
机构
[1] Natl Inst Stand & Technol, Biomat Grp, Div Polymers, Gaithersburg, MD 20899 USA
[2] Natl Inst Stand & Technol, Analyt Microscopy Grp, Surface & Microanal Sci Div, Gaithersburg, MD 20899 USA
[3] Rutgers State Univ, Dept Chem, New Jersey Ctr Biomat, Piscataway, NJ 08854 USA
关键词
biomaterials; fibronectin; tyrosine-derived polycarbonate; phase separation; real-time reverse transcriptase polymerase chain reaction (RT-PCR); interleukin-1; beta;
D O I
10.1002/jbm.a.30527
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Two-dimensional thin films consisting of homopolymer and discrete compositional blends of tyrosine-derived polycarbonates were prepared and characterized in an effort to elucidate the nature of different cell responses that were measured in vitro. The structurally similar blends were found to phase separate after annealing with domain sizes dependent on the overall composition. The thin polymer films were characterized with the use of atomic force microscopy (AFM), water contact angles, and time-of-flight secondary ion mass spectrometry (TOF-SIMS) and significant changes in roughness were measured following the annealing process. Genetic expression profiles of interleukin-1 beta and fibronectin in MC3T3-E1 osteoblasts and RAW 264.7 murine macrophages were measured at several time points, demonstrating the time and composition-dependent nature of the cell responses. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) depicted upregulation of the fibronectin gene copy numbers in each of the blends relative to the homopolymers. Moreover, the interleukin-1 beta expression profile was found to be compositionally dependent. The data suggest strongly that optimal composition and processing conditions can significantly affect the acute inflammatory and extracellular matrix production responses. (C) 2005 Wiley Periodicals, Inc.
引用
收藏
页码:491 / 502
页数:12
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