Urinary excretion of sulfated polysaccharides administered to Wistar rats suggests a renal permselectivity to these polymers based on molecular size

被引:21
作者
Guimaraes, MAM
Mourao, PAS
机构
[1] UNIV FED RIO DE JANEIRO, INST CIENCIAS BIOMED, DEPT BIOQUIM MED, BR-21941590 RIO DE JANEIRO, BRAZIL
[2] UNIV ESTADO RIO DE JANEIRO, DEPT MED INTERNA, RIO DE JANEIRO, BRAZIL
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1997年 / 1335卷 / 1-2期
关键词
sulfated polysaccharide; glycosaminoglycan; urinary excretion;
D O I
10.1016/S0304-4165(96)00133-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sulfated polysaccharides were administered to Wistar rats and their elimination from the blood as well as their urinary excretion were evaluated. Sulfated polysaccharides with differences in molecular mass, charge density and molecular structure were obtained from algae, marine invertebrates and vertebrates. A simple methodology based on the metachromatic property of these polysaccharides with 1,9-dimethylmethylene blue was used to estimate their concentration in urine and blood. Renal permselectivity to these macromolecules was based on molecular size, but the upper limit of molecular mass for excretion of a sulfated polysaccharide in urine varies among polymers with different structures. For dextran sulfates the upper limit is approximate to 8 kDa. Chondroitin 4- and 6-sulfates were excreted as fragments of approximate to 30 kDa, which is smaller than the injected polysaccharide. This suggests that they were degraded enzymatically in vivo. Large synthetic polymers (dextran sulfate > 8 kDa) were not excreted in urine, but slowly disappeared from the blood. Evaluation of their tissue distribution after intravenous administration indicated that these molecules are preferentially accumulated in the kidney.
引用
收藏
页码:161 / 172
页数:12
相关论文
共 33 条
[1]   ORAL DEXTRAN SULFATE (UA001) IN THE TREATMENT OF THE ACQUIRED IMMUNODEFICIENCY SYNDROME (AIDS) AND AIDS-RELATED COMPLEX [J].
ABRAMS, DI ;
KUNO, S ;
WONG, R ;
JEFFORDS, K ;
NASH, M ;
MOLAGHAN, JB ;
GORTER, R ;
UENO, R .
ANNALS OF INTERNAL MEDICINE, 1989, 110 (03) :183-188
[2]   SULFATED POLYSACCHARIDES ARE POTENT AND SELECTIVE INHIBITORS OF VARIOUS ENVELOPED VIRUSES, INCLUDING HERPES-SIMPLEX VIRUS, CYTOMEGALO-VIRUS, VESICULAR STOMATITIS-VIRUS, AND HUMAN IMMUNODEFICIENCY VIRUS [J].
BABA, M ;
SNOECK, R ;
PAUWELS, R ;
DECLERCQ, E .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1988, 32 (11) :1742-1745
[3]   DETERMINANTS OF GLOMERULAR PERMSELECTIVITY - INSIGHTS DERIVED FROM OBSERVATIONS INVIVO [J].
BRENNER, BM ;
BOHRER, MP ;
BAYLIS, C ;
DEEN, WM .
KIDNEY INTERNATIONAL, 1977, 12 (04) :229-237
[4]   PERMSELECTIVITY OF GLOMERULAR CAPILLARY WALL .3. RESTRICTED TRANSPORT OF POLYANIONS [J].
CHANG, RLS ;
DEEN, WM ;
ROBERTSON, CR ;
BRENNER, BM .
KIDNEY INTERNATIONAL, 1975, 8 (04) :212-218
[5]   IMPROVED QUANTITATION AND DISCRIMINATION OF SULFATED GLYCOSAMINOGLYCANS BY USE OF DIMETHYLMETHYLENE BLUE [J].
FARNDALE, RW ;
BUTTLE, DJ ;
BARRETT, AJ .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 883 (02) :173-177
[6]   CRYSTAL INHIBITION - THE EFFECTS OF POLYANIONS ON CALCIUM-OXALATE CRYSTAL-GROWTH [J].
FELLSTROM, B ;
DANIELSON, BG ;
LJUNGHALL, S ;
WIKSTROM, B .
CLINICA CHIMICA ACTA, 1986, 158 (03) :229-235
[7]   TREATMENT WITH A GLYCOSAMINOGLYCAN FORMULATION AMELIORATES EXPERIMENTAL DIABETIC NEPHROPATHY [J].
GAMBARO, G ;
VENTURINI, AP ;
NOONAN, DM ;
FRIES, W ;
RE, G ;
GARBISA, S ;
MILANESI, C ;
PESARINI, A ;
BORSATTI, A ;
MARCHI, E ;
BAGGIO, B .
KIDNEY INTERNATIONAL, 1994, 46 (03) :797-806
[8]   DEGRADATION OF HYALURONIC-ACID BY POLYMORPHONUCLEAR LEUKOCYTES [J].
GREENWALD, RA ;
MOAK, SA .
INFLAMMATION, 1986, 10 (01) :15-30
[9]  
GRIFFITH MJ, 1982, J BIOL CHEM, V257, P7360
[10]   ACRYLAMIDE GEL ELECTROPHORESIS OF ACIDIC MUCOPOLYSACCHARIDES [J].
HILBORN, JC ;
ANASTASSIADIS, PA .
ANALYTICAL BIOCHEMISTRY, 1969, 31 (1-3) :51-+