Molecular cloning and characterization of a novel low temperature-induced gene, blti2, from barley (Hordeum vulgare L.)

被引:12
作者
Bahn, SC
Bae, MS
Park, YB
Oh, SI
Jeung, JU
Bae, JM
Chung, YS
Shin, JS [1 ]
机构
[1] Korea Univ, Grad Sch Biotechnol, Seoul 136701, South Korea
[2] Dong A Univ, Life Sci & Nat Resources, Pusan 604714, South Korea
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2001年 / 1522卷 / 02期
关键词
barley (hordeum vulgare L. cv. Dongbori); differential gene expression; low temperature induction; blti2; suppression subtractive hybridization;
D O I
10.1016/S0167-4781(01)00317-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Suppression Subtractive Hybridization (SSH) was used to isolate large numbers of low temperature-induced genes from the cold-treated winter barley (Hordeum vulgare L. cv. Dongbori). One hundred and sixty blti (barley low temperature-induced) cDNA clones were obtained. Northern blot analyses showed that several blti clones were differentially expressed by treatment of low, temperature, NaCl, dehydration and ABA. One of the clones, blti2, was induced from 3 to 72 h after cold treatment while its transcript was detected only at 12 h after ABA treatment, indicating that the expression of blti2 by low temperature was regulated by an ABA-independent pathway. The full-length cDNA sequences were 944 nucleotides long and the open reading frame consisted of 492 nucleotides encoding 164 amino acids. Nucleotide sequences showed no sequence homology with the previously reported low temperature-responsive (LTR) barley genes, and the deduced amino acid sequences revealed that the blti2 contains three membrane-spanning regions. These results suggest that blti2 is a novel transmembrane protein induced by low temperature. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:134 / 137
页数:4
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