Survival of cells and DNA of Aeromonas salmonicida released into aquatic microcosms

被引:36
作者
Deere, D
Porter, J
Pickup, RW
Edwards, C
机构
[1] UNIV LIVERPOOL, DEPT GENET & MICROBIOL, LIVERPOOL L69 3BX, MERSEYSIDE, ENGLAND
[2] INST FRESHWATER ECOL, WINDERMERE LABS, AMBLESIDE LA22 0LP, CUMBRIA, ENGLAND
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1996年 / 81卷 / 03期
关键词
D O I
10.1111/j.1365-2672.1996.tb04333.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The survival of the bacterial fish pathogen Aeromonas salmonicida, and persistence of its DNA, were monitored in aquatic microcosms using selective culture and most probable number PCR. Bacterial cells and naked DNA were released into natural non-sterile microcosms consisting of lake sediment overlayered with lake water. Two different types of surface sediment were used. One was sandy in character, taken from the shoreline whilst the other was a littoral loamy surface mud. Inoculated cells and naked DNA became undetectable from water overlayers within 4 weeks of release. Colony counts of Aer. salmonicida declined below detectable limits after 4 weeks in loamy sediment or 7 weeks in sandy sediment; however, naked DNA and DNA from released cells remained detectable for more than 13 weeks.
引用
收藏
页码:309 / 318
页数:10
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