PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) regulates auxin biosynthesis at high temperature

被引:549
作者
Franklin, Keara A. [1 ]
Lee, Sang Ho [2 ]
Patel, Dhaval [1 ]
Kumar, S. Vinod [3 ]
Spartz, Angela K. [2 ]
Gu, Chen [4 ]
Ye, Songqing [5 ]
Yu, Peng [2 ]
Breen, Gordon [1 ]
Cohen, Jerry D. [5 ,6 ]
Wigge, Philip A. [3 ]
Gray, William M. [2 ,6 ]
机构
[1] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England
[2] Univ Minnesota, Dept Plant Biol, St Paul, MN 55108 USA
[3] John Innes Ctr, Dept Cell & Dev Biol, Norwich NR4 7UH, Norfolk, England
[4] Macalester Coll, Dept Biol, St Paul, MN 55105 USA
[5] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA
[6] Univ Minnesota, Microbial & Plant Genom Inst, St Paul, MN 55108 USA
基金
英国生物技术与生命科学研究理事会; 美国国家科学基金会; 美国国家卫生研究院; 欧洲研究理事会;
关键词
indole-3-acetic acid; TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1; CYP79B2; SHADE AVOIDANCE; ARABIDOPSIS-THALIANA; PLANT DEVELOPMENT; CIRCADIAN CLOCK; HYPOCOTYL ELONGATION; TRANSCRIPTION FACTOR; GROWTH; GENES; LIGHT; INHIBITION;
D O I
10.1073/pnas.1110682108
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
At high ambient temperature, plants display dramatic stem elongation in an adaptive response to heat. This response is mediated by elevated levels of the phytohormone auxin and requires auxin biosynthesis, signaling, and transport pathways. The mechanisms by which higher temperature results in greater auxin accumulation are unknown, however. A basic helix-loop-helix transcription factor, PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), is also required for hypocotyl elongation in response to high temperature. PIF4 also acts redundantly with its homolog, PIF5, to regulate diurnal growth rhythms and elongation responses to the threat of vegetative shade. PIF4 activity is reportedly limited in part by binding to both the basic helix-loop-helix protein LONG HYPOCOTYL IN FAR RED 1 and the DELLA family of growth-repressing proteins. Despite the importance of PIF4 in integrating multiple environmental signals, the mechanisms by which PIF4 controls growth are unknown. Here we demonstrate that PIF4 regulates levels of auxin and the expression of key auxin biosynthesis genes at high temperature. We also identify a family of SMALL AUXIN UP RNA (SAUR) genes that are expressed at high temperature in a PIF4-dependent manner and promote elongation growth. Taken together, our results demonstrate direct molecular links among PIF4, auxin, and elongation growth at high temperature.
引用
收藏
页码:20231 / 20235
页数:5
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