Subchronic toxicity study of GH transgenic carp

被引：13

作者：

Yong, Ling ^{[1
]}

Liu, Yu-Mei ^{[2
]}

Jia, Xu-Dong ^{[1
]}

Li, Ning ^{[1
]}

Zhang, Wen-Zhong ^{[1
]}

机构：

[1] China CDC, Natl Inst Nutr & Food Safety, Beijing 100021, Peoples R China

[2] Beijing Union Univ, Coll Appl Arts & Sci, Beijing 100191, Peoples R China

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2012年 / 50卷 / 11期

关键词：

Growth hormone transgenic carp; Subchronic toxicity; Sprague-Dawley rat; Endocrine disruption; ECOLOGICAL RISK; FISH; GROWTH; SELECTION; PROGRESS; RELEASE; SALMON;

D O I：

10.1016/j.fct.2012.07.064

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A subchronic toxicity study of GH (growth hormone) transgenic carp was carried out with 60 SD rats aged 4 weeks, weight 115 similar to 125 g. Ten male and 10 female rats were allotted into each group. Animals of the three groups (transgenic carp group (GH-TC), parental carp group (PC) and control group) were fed soy- and alfalfa-free diet (SAFD) with 10% GH transgenic carp powder, 10% parental carp powder or 10% common carp powder for 90 consecutive days, respectively. In the end of study, animals were killed by exsanguination via the carotid artery under diethyl ether anesthesia, then weights of heart, liver, kidneys, spleen, thymus, brain, ovaries and uterus/testis were measured. Pathological examination of organs was determined. Endocrine hormones of triiodothyronine (T3), thyroid hormone (T4), follicle-stimulating hormone (FSH), 17 beta-estradiol (E2), progesterone (P) and testosterone (T) levels were detected by specific ELISA kit. Parameters of blood routine and blood biochemical were measured. The weights of the body and organs of the rats, food intake, blood routine, blood biochemical test and serum hormones showed no significant differences among the GH transgenic carp-treated, parental carp-treated and control groups (P > 0.05). Thus, it was concluded that at the dose level of this study, CH transgenic carp showed no subchronic toxicity and endocrine disruption to SD rats. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：3920 / 3926

页数：7

共 38 条

[1]

[Anonymous], 1985, J APPL ICHTHYOL, DOI DOI 10.1111/J.1439-0426.1985.TB00408.X

[2] Subchronic feeding study of grain from herbicide-tolerant maize DP-empty set9814empty set-6 in Sprague-Dawley rats [J].

Appenzeller, Laura M. ;

Munley, Susan M. ;

Hoban, Denise ;

Sykes, Greg P. ;

Malley, Linda A. ;

Delaney, Bryan .

FOOD AND CHEMICAL TOXICOLOGY, 2009, 47 (09) :2269-2280

[3] Subchronic feeding study with genetically modified stacked trait lepidopteran and coleopteran resistant (DAS-empty set 15empty set 07-1xDAS-59122-7) maize grain in Sprague-Dawley rats [J].

Appenzeller, Laura M. ;

Malley, Linda ;

MacKenzie, Susan A. ;

Hoban, Denise ;

Delaney, Bryan .

FOOD AND CHEMICAL TOXICOLOGY, 2009, 47 (07) :1512-1520

[4]

Cao D.C., 1998, CHIN HIGH TECHNOL LE, V3, P45

[5]

Chen KaiJian Chen KaiJian, 2002, Journal of Hunan Agricultural University, V28, P147

[6]

Chen T T, 1996, Biotechnol Annu Rev, V2, P205, DOI 10.1016/S1387-2656(08)70011-5

[7]

de Vendômois JS, 2010, INT J BIOL SCI, V6, P590

[8]

de Vendômois JS, 2009, INT J BIOL SCI, V5, P706

[9] Interface of biotechnology and ecology for environmental risk assessments of transgenic fish [J].

Devlin, RH ;

Sundström, LF ;

Muir, WM .

TRENDS IN BIOTECHNOLOGY, 2006, 24 (02) :89-97

[10] EXTRAORDINARY SALMON GROWTH [J].

DEVLIN, RH ;

YESAKI, TY ;

BIAGI, CA ;

DONALDSON, EM ;

SWANSON, P ;

CHAN, WK .

NATURE, 1994, 371 (6494) :209-210

← 1 2 3 4 →