Protective effect of Clostridium septicum alpha-toxoid vaccine against challenge with spores in guinea pigs

被引：21

作者：

Amimoto, K ^{[1
]}

Ohgitani, T ^{[1
]}

Sasaki, O ^{[1
]}

Oishi, E ^{[1
]}

Katayama, S ^{[1
]}

Isogai, M ^{[1
]}

Ota, S ^{[1
]}

机构：

[1] Kyoto Biken Labs, Div Vet Microbiol, Uji, Kyoto 6110041, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2002年 / 64卷 / 01期

关键词：

alpha-toxin; Clostridium septicum; toxoid;

D O I：

10.1292/jvms.64.67

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The protective effect of an alpha-toxoid vaccine of Clostridium septicum purified alpha-toxin was investigated in guinea pigs. Purified alpha-toxin was treated with formalin to make toxoid, and alpha-toxoid vaccine was prepared by mixing alpha-toxoid (4 to 64 mug/dose) with an aluminum phosphate gel as adjuvant. Guinea pigs were immunized twice with different doses of alpha-toxoid vaccine, and challenged with spores of C. septicum. The guinea pigs surviving after challenge had been immunized with 8 mug/dose or more of alpha-toxoid. All these animals produced titers of 20 units or higher of antitoxin at the challenge. The results suggest that C. septicum alpha-toxin plays an important role in protection against challenge with spores in guinea pigs.

引用

页码：67 / 69

页数：3

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