Expression of a soluble flavone synthase allows the biosynthesis of phytoestrogen derivatives in Escherichia coli

被引:64
作者
Leonard, E [1 ]
Chemler, J [1 ]
Lim, KH [1 ]
Koffas, MAG [1 ]
机构
[1] SUNY Buffalo, Dept Biol & Chem Engn, Buffalo, NY 14260 USA
基金
美国国家科学基金会;
关键词
D O I
10.1007/s00253-005-0059-x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Flavones are plant secondary metabolites with potent pharmacological properties. We report the functional expression of FSI, a flavonoid 2-oxoglutarate-dependent dioxygenase-encoding flavone synthase from parsley in Escherichia coli. This expression allows the biosynthesis of various flavones from phenylpropanoid acids in recombinant E. coli strains simultaneously expressing five plant-specific flavone biosynthetic genes. The gene ensemble consists of 4CL-2 (4-coumarate:CoA ligase) and FSI (flavone synthase I) from parsley, chsA (chalcone synthase) and chiA (chalcone isomerase) from Petunia hybrida, and OMT1A (7-O-methyltransferase) from peppermint. After a 24-h cultivation, the recombinant E. coli produces significant amounts of apigenin (415 mu g/l), luteolin (10 mu g/l), and genkwanin (208 mu g/l). The majority of the flavone products are excreted in the culture media; however, 25% is contained within the cells. The metabolic engineering strategy presented demonstrates that plant-specific flavones are successfully produced in E. coli for the first time by incorporating a soluble flavone synthase confined only in Apiaceae.
引用
收藏
页码:85 / 91
页数:7
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