Rapid detection of several foodborne pathogens by F0F1-ATPase molecular motor biosensor

被引：12

作者：

Zhang, Jie ^{[1
]}

Li, Zhaojie ^{[2
]}

Zhang, Huiyuan ^{[1
]}

Wang, Jing ^{[2
]}

Liu, Yan ^{[1
]}

Chen, Guangquan ^{[1
]}

机构：

[1] Beijing Entry Exit Inspect & Quarantine Bur, Beijing 100026, Peoples R China

[2] Weihai Entry Exit Inspect & Quarantine Bur, Weihai 264205, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2013年 / 93卷 / 01期

关键词：

F0F1-ATPase; Molecular motor biosensor; ATP; Detection; Foodborne pathogens; ATP SYNTHASE; GOLD NANOPARTICLES; BIOTERRORISM; TRANSPORT; MACHINE; DRIVEN;

D O I：

10.1016/j.mimet.2013.01.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

F0F1-ATPase within chromatophore was constructed as a molecular motor biosensor through epsilon-subunit antibody-biotin-streptavidin-biotin-AC5-Sulfo-Osu system. Based on probe-DNA specific binding, DNA of several foodborne pathogens Listeria monocytogenes, Salmonella typhimurium, Vibrio parahaemolyticus and Vibrio cholerae was specifically captured by F0F1-ATPase molecular motor biosensors. Loads of DNA decreased the rotation rate of F0F1-ATPase, and led to the decrease of ATP synthesis. The detection of pathogens based on proton flux change driven by ATP-synthesis of F0F1-ATPase, which was indicated by F-DHPE, was monitored by a fluorescence spectrometer. The results demonstrate that the F0F1-ATPase molecular motor biosensor can specifically detect bacterial DNA at low concentration level, and will be a convenient, quick, and promising tool for detecting pathogens. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：37 / 41

页数：5

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