A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses

被引：21

作者：

Lindau, Cecilia ^{[1
,2
]}

Tiveljung-Lindell, Annika ^{[1
,2
]}

Goha, Shan ^{[1
,2
]}

Ramqvist, Torbjorn ^{[3
]}

Allander, Tobias ^{[1
,2
]}

机构：

[1] Karolinska Univ Hosp, Dept Clin Microbiol, SE-17176 Stockholm, Sweden

[2] Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden

[3] Karolinska Inst, Dept Pathol & Oncol, Stockholm, Sweden

来源：

JOURNAL OF CLINICAL VIROLOGY | 2009年 / 44卷 / 01期

基金：

瑞典研究理事会;

关键词：

KI polyomavirus; WU polyomavirus; Real-time PCR; Nasopharyngeal aspirates; Diagnostics; RESPIRATORY-TRACT INFECTIONS; BK VIRUS; CHILDREN; DISEASE; DNA;

D O I：

10.1016/j.jcv.2008.09.006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Three new human polyomaviruses have been recently described, and investigating their in vivo biology and pathogenicity will require sensitive and rational detection assays. Objectives: To develop and evaluate a sensitive and rational assay for detection of the newly identified KI and WU polyomaviruses. Study design: A single-tube, dual-probe, real-time PCR assay for simultaneous detection and discrimination of KI and WU polyomaviruses was developed. Results: The assay had near single-molecule sensitivity for both viruses and no cross-reactivity was observed. A panel of 637 nasopharyngeal aspirates was screened, resulting in a frequency of 1.4% for KlPyV and 1.3% for WUPyV. Conclusions: The dual-probe assay provides a rational approach for further studies of KlPyV and WUPyV. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：24 / 26

页数：3

共 20 条

[1] WU polyomavirus in children, Canada [J].

Abed, Yacine ;

Wang, David ;

Boivin, Guy .

EMERGING INFECTIOUS DISEASES, 2007, 13 (12) :1939-1941

[2]

Ahsan N, 2006, ADV EXP MED BIOL, V577, P1

[3] Identification of a third human polyomavirus [J].

Allander, Tobias ;

Andreasson, Kalle ;

Gupta, Shawon ;

Bjerkner, Annelie ;

Bogdanovic, Gordana ;

Persson, Mats A. A. ;

Dalianis, Tina ;

Ramqvist, Torbjorn ;

Andersson, Bjorn .

JOURNAL OF VIROLOGY, 2007, 81 (08) :4130-4136

[4] Development and evaluation of real-time PCR assays for the detection of the newly identified KI and WU polyomaviruses [J].

Bialasiewicza, S. ;

Whiley, D. M. ;

Lambert, S. B. ;

Gould, A. ;

Nissen, M. D. ;

Sloots, T. P. .

JOURNAL OF CLINICAL VIROLOGY, 2007, 40 (01) :9-14

[5]

Feng HC, 2008, SCIENCE, V319, P1096, DOI 10.1126/science.1152586

[6] KI and WU polyomaviruses in children, France [J].

Foulongne, Vincent ;

Brieu, Natalie ;

Jeziorski, Eric ;

Chatain, Amandine ;

Rodiere, Michel ;

Segondy, Michel .

EMERGING INFECTIOUS DISEASES, 2008, 14 (03) :523-525

[7] Identification of a novel polyomavirus from patients with acute respiratory tract infections [J].

Gaynor, Anne M. ;

Nissen, Michael D. ;

Whiley, David M. ;

Mackay, Ian M. ;

Lambert, Stephen B. ;

Wu, Guang ;

Brennan, Daniel C. ;

Storch, Gregory A. ;

Sloots, Theo P. ;

Wang, David .

PLOS PATHOGENS, 2007, 3 (05) :595-604

[8] THE ROLE OF BK VIRUS IN ACUTE RESPIRATORY-TRACT DISEASE AND THE PRESENCE OF BKV DNA IN TONSILS [J].

GOUDSMIT, J ;

WERTHEIMVANDILLEN, P ;

VANSTRIEN, A ;

VANDERNOORDAA, J .

JOURNAL OF MEDICAL VIROLOGY, 1982, 10 (02) :91-99

[9] WU polyomavirus in children with acute lower respiratory tract infections, South Korea [J].

Han, Tae Hee ;

Chung, Ju-Young ;

Koo, Ja Wook ;

Kim, Sang Woo ;

Hwang, Eung-Soo .

EMERGING INFECTIOUS DISEASES, 2007, 13 (11) :1766-1768

[10]

KIASARI BA, 2008, J CLIN VIROL

← 1 2 →