Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria

被引：120

作者：

Pelicic, V

Reyrat, JM

Gicquel, B

机构：

[1] U. de Genet. Mycobacterienne, Ctr. Natl. de la Rech. Sci. URA 1300, Institut Pasteur

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 04期

关键词：

D O I：

10.1128/jb.178.4.1197-1199.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilislis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycobacteria.

引用

页码：1197 / 1199

页数：3

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