Correlation between microdilution, E-test, and disk diffusion methods for antifungal susceptibility testing of posaconazole against Candida spp.

被引:33
作者
Sims, Charles R. [1 ]
Paetznick, Victor L. [1 ]
Rodriguez, Jose R. [1 ]
Chen, Enuo [1 ]
Ostrosky-Zeichner, Luis [1 ]
机构
[1] Univ Texas, Sch Med, Div Infect Dis, Lab Mycol Res, Houston, TX 77030 USA
关键词
D O I
10.1128/JCM.02591-05
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Agar-based antifungal susceptibility testing is an attractive alternative to the microdilution method. We examined the correlation between the microdilution, E-test, and disk diffusion methods for posaconazole against Candida spp. A total of 270 bloodstream isolates of Candida spp. with a broad range of posaconazole MICs were tested using the CLSI M27-A2 method for microdilution, as well as the M-44A method and E-test methods for agar-based testing on Mueller-Hinton agar supplemented with 2% glucose and 0.5 mu g of methylene blue. MICs and inhibitory zone diameters at the prominent growth reduction endpoint were recorded at 24 and 48 h. The Candida isolates included Candida albicans (n = 124), C. parapsilosis (n = 44), C. tropicalis (n = 41), C. glabrata (n = 36), C. krusei (n = 20), C. lusitaniae (n = 3), and C. dubliniensis (n = 2). The overall concordance (i.e., the percentage of isolates within two dilutions) between the E-test and microdilution was 64.8% at 24 h and 82.6% at 48 h. When we considered an arbitrary breakpoint of <= 1 mu g/ml, the agreement between the E-test and microdilution methods was 87.8% at 24 h and 93.0% at 48 h. The correlation of MICs with disk diffusion zone diameters was better for the E-test than the microdilution method. Zone correlation for diameters produced by the disks of two manufacturers was high, with a Pearson test value of 0.941 at 24 h. The E-test and microdilution MICs show good concordance and interpretative agreement. The disk diffusion zone diameters are highly reproducible and correlate well with both the E-test and the microdilution method, making agar-based methods a viable alternative to microdilution for posaconazole susceptibility testing.
引用
收藏
页码:2105 / 2108
页数:4
相关论文
共 17 条
[1]   Comparison of visual and spectrophotometric methods of broth microdilution MIC end point determination and evaluation of a sterol quantitation method for in vitro susceptibility testing of fluconazole and itraconazole against trailing and nontrailing Candida isolates [J].
Arthington-Skaggs, BA ;
Lee-Yang, W ;
Ciblak, MA ;
Frade, JP ;
Brandt, ME ;
Hajjeh, RA ;
Harrison, LH ;
Sofair, AN ;
Warnock, DW .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (08) :2477-2481
[2]   Fluconazole disk diffusion procedure for determining susceptibility of Candida species [J].
Barry, AL ;
Brown, SD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (09) :2154-2157
[3]  
*CLIN LAB STAND I, 1999, M23A2 CLSI
[4]  
Clinical and Laboratory Standards Institute, 2002, M44A CLSI
[5]  
Clinical and Laboratory Standards Institute, 2002, M27A2 CLSI
[6]   Multicenter comparison of the sensititre YeastOne Colorimetric Antifungal Panel with the NCCLS M27-A2 reference method for testing new antifungal agents against clinical isolates of Candida spp. [J].
Espinel-Ingroff, A ;
Pfaller, M ;
Messer, SA ;
Knapp, CC ;
Holliday, N ;
Killian, SB .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (02) :718-721
[7]  
Gleason TG, 1997, ARCH SURG-CHICAGO, V132, P1197, DOI 10.1001/archsurg.1997.01430350047008
[8]   Non-albicans Candida spp. causing fungaemia:: pathogenicity and antifungal resistance [J].
Krcmery, V ;
Barnes, AJ .
JOURNAL OF HOSPITAL INFECTION, 2002, 50 (04) :243-260
[9]   Correlation between E-test, disk diffusion, and microdilution methods for antifungal susceptibility testing of fluconazole and voriconazole [J].
Matar, MJ ;
Ostrosky-Zeichner, L ;
Paetznick, VL ;
Rodriguez, JR ;
Chen, E ;
Rex, JH .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (05) :1647-1651
[10]   SUSCEPTIBILITY TESTS OF ANAEROBIC BACTERIA - STATISTICAL AND CLINICAL CONSIDERATIONS [J].
METZLER, CM ;
DEHAAN, RM .
JOURNAL OF INFECTIOUS DISEASES, 1974, 130 (06) :588-594