Evaluation of LabChip™ technology for GMO analysis in food

被引:28
作者
Birch, L [1 ]
Archard, CL [1 ]
Parkes, HC [1 ]
McDowell, DG [1 ]
机构
[1] LGC, Life Sci Res Dept, Teddington TW11 0LW, Middx, England
关键词
GM food; LabChip (TM) technology; quantitative PCR;
D O I
10.1016/S0956-7135(01)00062-7
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Ongoing technological advances permit the development of new scientific strategies for bio-analysis. The work described here sought to compare a traditional agarose slab gel electrophoresis method combined with digital image analysis against a new microfluidic capillary electrophoresis system (LabChip (TM), Agilent Technologies) to assess their relative performance in the quantitative analysis of polymerase chain reaction (PCR) products. The comparative strategy exploited methodology for providing quantitative genetically modified organism (GMO) determinations in food samples and demonstrated that the LabChip (TM) system offered improvements in quantitation accuracy, objectivity and ease of use. (C) 2001 LGC (Teddington) Ltd. Published by Elsevier Science Ltd. All rig ts reserved.
引用
收藏
页码:535 / 540
页数:6
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