A novel toxin from the venom of the scorpion Tityus trivittatus, is the first member of a new α-KTX subfamily

被引：29

作者：

Abdel-Mottaleb, Y

Coronas, FV

de Roodt, AR

Possani, LD

Tytgat, J

机构：

[1] Katholieke Univ Leuven, Toxicol Lab, B-3000 Louvain, Belgium

[2] Natl Autonomous Univ Mexico, Inst Biotechnol, Dept Mol Med & Bioproc, Cuernavaca 66210, Morelos, Mexico

[3] Natl Inst Prod Biol, Adm Nacl Labs, Buenos Aires, DF, Argentina

[4] Inst Salud Dr Carlos G Malbran, Buenos Aires, DF, Argentina

来源：

FEBS LETTERS | 2006年 / 580卷 / 02期

关键词：

Tityus trivittatus; alpha-KTx20.1; voltage-gated K+ channels;

D O I：

10.1016/j.febslet.2005.12.073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The first example of a new sub-family of toxins (alpha-KTx20.1) from the scorpion Tityus trivittatus was purified, sequenced and characterized physiologically. It has 29 amino acid residues, three disulfide bridges assumed to adopt the cysteine-stabilized alpha/beta scaffold with a pI value of 8.98. The sequence identities with all the other known alpha-KTx are less than 40%. Its effects were verified using seven different cloned K+ channels (vertebrate Kv1.1-1.5, Shaker IR and hERG) expressed in Xenopus leavis oocytes. The toxin-induced effects show large differences among the different K+ channels and a preference towards Kv1.3 (EC50 = 7.9 +/- 1.4 nM). (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：592 / 596

页数：5

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