In vitro evaluation of oxidative damage from organic solvent vapours on human skin

Aim of this study is to focus on the cutaneous effects of occupational exposure to the vapours of common organic solvents. Higher incidence of cancer is suspected in subjects exposed to organic solvents characterized by reactive metabolic intermediates, which are assumed to induce oxidative damage on liver, kidney and haematopoietic system. To our knowledge there are no relevant data about the oxidative effect of the organic solvents on skin after exposure to their vapours. An in vitro model was optimized to evaluate the viability and integrity of the barrier function of human skin following occupational exposure to solvent vapours. To evaluate the role of oxidative stress, the antioxidant status of skin and some biomarkers of oxidative damage as lipid and protein peroxidation products and DNA fragmentation were also studied. It was investigated if decreasing concentrations of styrene, toluene, acetone, xylene and perchloroethylene vapours, up to be comparable to the TLV-TWA, were able to damage skin integrity. Solvent vapours reduced tissue viability and impaired skin barrier function, as indicated by the increase of transepidermal water loss. Reduced glutathione depletion, decreased activity of antioxidant enzymes and oxidative damage of biological macromolecules were also observed. The overall trend of these results indicates that the tested compounds damage human skin even at concentrations comparable to their TLV, and that oxidative stress is involved in these effects. (c) 2005 Elsevier Ltd. All rights reserved.