Structural characterization of bioactive pectic polysaccharides from elderflowers (Sambuci flos)

被引：69

作者：

Giang Thanh Thi Ho ^{[1
]}

Zou, Yuan-Feng ^{[1
]}

Aslaksen, Torun Helene ^{[1
]}

Wangensteen, Helle ^{[1
]}

Barsett, Hilde ^{[1
]}

机构：

[1] Univ Oslo, Sch Pharm, Dept Pharmaceut Chem, N-0316 Oslo, Norway

来源：

CARBOHYDRATE POLYMERS | 2016年 / 135卷

关键词：

Sambucus nigra; Elderflower; Immunological activity; Pectic polysaccharides; Complement system; Lipopolysaccharide; RHAMNOGALACTURONAN-II; QUANTITATIVE-ANALYSIS; OPILIA-CELTIDIFOLIA; PLANT; ACID; ARABINOGALACTAN; COMPLEMENT; EXTRACTS; ELDER; L;

D O I：

10.1016/j.carbpol.2015.08.056

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Elderflowers have traditionally been used and are still used for its anti-inflammatory property. Traditionally elderflowers were used as remedies against cold, flu and diuretic. The aim of this study was to relate the structure of pectic-polysaccharides from elderflowers to immunomodulating properties. Purified fractions obtained by gelfiltration and ion exchange chromatography of 50% ethanol, 50 degrees C water and 100 degrees C water extracts exhibited strong complement fixating activity and macrophage stimulating activity. Reduced bioactivity was observed after removal of arabinose and 1,3,6-Gal linkages by weak acid hydrolysis. Enhanced bioactivity was observed after removal of estergroups by NaOH. Relating linkage analysis to the results of the bioactivity tests, led to the assumption that the branched moieties of the arabinogalactans linked to rhamnogalacturonan region, is important for the immunomodulating activity seen in elderflowers. No cytotoxity was observed. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：128 / 137

页数：10

共 57 条

[1]

[Anonymous], 2000, HERBAL MED EXPANDED

[2]

Anti-ulcer polysaccharides from Cola cordifolia bark and leaves [J].