Differentiation of Ehrlichia platys and E-equi infections in dogs by using 16S ribosomal DNA-based PCR

被引:15
作者
Hancock, SI [1 ]
Breitschwerdt, EB [1 ]
Pitulle, C [1 ]
机构
[1] N Carolina State Univ, Coll Vet Med, Dept Clin Sci, Raleigh, NC 27606 USA
关键词
D O I
10.1128/JCM.39.12.4577-4578.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have encountered a previously unrecognized specificity problem when using the small-subunit ribosomal DNA (16S rDNA)-based PCR primers recommended for use in the identification of Ehrlichia equi in clinical samples. These PCR primers annealed to E. platys 16S rDNA in blood samples containing high levels of E. platys organisms. Therefore, we designed and tested new PCR primers for the identification of E. equi.
引用
收藏
页码:4577 / 4578
页数:2
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