Elicitor and Fusarium-induced expression of NPR1-like genes in banana

被引：64

作者：

Endah, Rosita ^{[2
,3
]}

Beyene, Getu

Kiggundu, Andrew ^{[4
]}

van den Berg, Noelani ^{[3
,5
]}

Schluter, Urte ^{[2
,3
]}

Kunert, Karl ^{[2
,3
]}

Chikwamba, Rachel ^{[1
,2
,3
]}

机构：

[1] CSIR, Biosci Div, ZA-0001 Pretoria, South Africa

[2] Univ Pretoria, Dept Plant Sci, ZA-0002 Pretoria, South Africa

[3] Univ Pretoria, Forestry & Agr Biotechnol Inst, ZA-0002 Pretoria, South Africa

[4] NARO, Entebbe, Uganda

[5] Univ Pretoria, Dept Microbiol & Plant Pathol, ZA-0002 Pretoria, South Africa

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2008年 / 46卷 / 11期

关键词：

NPR1; Banana; Musa; Fusarium oxysporum; Systemic acquired resistance; PR proteins;

D O I：

10.1016/j.plaphy.2008.06.007

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The non-expressor of pathogenesis-related genes 1 (NPR1) is an essential positive regulator of salicylic acid (SA)-induced pathogenesis-related (PR) gene expression and systemic acquired resistance (SAR). Two novel full length NPR1-like genes: MNPR1A and MNPR1B, were isolated from banana by application of the PCR and rapid amplification of cDNA ends (PACE) techniques. The two identified MNPR1 sequences differed greatly in their expression profile using quantitative real time (qRT)-PCR following either elicitor or Fusarium oxysporum Schlecht f. sp. cubense (Smith) Snyd (Foc) treatment. MNPR1A was greatly expressed after Foc treatment with higher and earlier expression in the Foc-tolerant cultivar GCTCV-218 than in the sensitive cultivar Grand Naine. In comparison, MNPR1B was highly responsive to SA, but not to methyl jasmonate (MeJA) treatment, in both the tolerant banana cultivar GCTCV-218 and the more sensitive cultivar Grand Naine. Expression of the MNPR1 genes further directly related to PR gene expression known to be involved in fungal resistance. Reduced sensitivity to Foc in GCTCV-218 might be partially attributed to the higher and an earlier expression of both MNPR1A and PR-1 in this cultivar after Foc treatment. Further characterisation of the MNPR1 genes through complementation of Arabidopsis npr1 mutants and overexpression studies in banana cultivars is the subject of ongoing and future work. (C) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：1007 / 1014

页数：8

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