The carboxyl-terminal sequence of the human secretory mucin, MUC6 - Analysis of the primary amino acid sequence

被引:91
作者
Toribara, NW
Ho, SB
Gum, JR
Lau, P
Kim, YS
机构
[1] UNIV CALIF SAN FRANCISCO, DIV GASTROENTEROL, SAN FRANCISCO, CA 94121 USA
[2] UNIV MINNESOTA, DEPT MED, MINNEAPOLIS, MN 55417 USA
[3] DEPT VET AFFAIRS MED CTR, MINNEAPOLIS, MN 55417 USA
关键词
D O I
10.1074/jbc.272.26.16398
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The distribution of MUC6 suggests that its primary function is protection of vulnerable epithelial surfaces from damaging effects of constant exposure to a wide range of endogenous caustic or proteolytic agents. A combination of genomic, cDNA. and 3' rapid amplification of cDNA ends techniques was used to isolate the carboxyl-terminal end of MUC6. The 3' nontandem repeat region contained 1083 base pairs of coding sequence (361 amino acids) followed by 632 base pairs of 3'-untranslated region. The coding sequence consists of two distinct regions; region 1 contained the initial 270 amino acids (62% Ser-Thr-Pro with no Cys residues), and region 2 contained the COOH-terminal 91 amino acids (22% Ser-Thr-Pro with 12% Cys). Although region 1 had no homology to any sequences in GenBank, region 2 had approximately 25% amino acid homology to the COOH-terminal regions of human mucins MUC2, -5, and -5B and von Willebrand factor. The shortness of region 2 would leave little of the peptide backbone exposed to a potentially hostile environment. Antibody studies suggest that MUC6 in its native form exists as a disulfide-bonded multimer, The conservation of the 11 cysteine positions in region 2 suggests the importance of this short region to mucin polymerization.
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页码:16398 / 16403
页数:6
相关论文
共 45 条
[1]   EVIDENCE FOR DIFFERENT HUMAN TRACHEOBRONCHIAL MUCIN PEPTIDES DEDUCED FROM NUCLEOTIDE CDNA SEQUENCES [J].
AUBERT, JP ;
PORCHET, N ;
CREPIN, M ;
DUTERQUECOQUILLAUD, M ;
VERGNES, G ;
MAZZUCA, M ;
DEBUIRE, B ;
PETITPREZ, D ;
DEGAND, P .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1991, 5 (02) :178-185
[2]   GENERAL METHOD FOR ISOLATION OF HIGH MOLECULAR-WEIGHT DNA FROM EUKARYOTES [J].
BLIN, N ;
STAFFORD, DW .
NUCLEIC ACIDS RESEARCH, 1976, 3 (09) :2303-2308
[3]  
BOBEK LA, 1993, J BIOL CHEM, V268, P20563
[4]   DEGLYCOSYLATION OF MUCIN FROM LS174T COLON CANCER-CELLS BY HYDROGEN-FLUORIDE TREATMENT [J].
BYRD, JC ;
LAMPORT, DTA ;
SIDDIQUI, B ;
KUAN, SF ;
ERICKSON, R ;
ITZKOWITZ, SH ;
KIM, YS .
BIOCHEMICAL JOURNAL, 1989, 261 (02) :617-625
[5]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[6]  
CONG NV, 1990, HUM GENET, V86, P167
[7]   MUC6 APOMUCIN SHOWS A DISTINCT NORMAL TISSUE DISTRIBUTION THAT CORRELATES WITH LEWIS ANTIGEN EXPRESSION IN THE HUMAN STOMACH [J].
DEBOLOS, C ;
GARRIDO, M ;
REAL, FX .
GASTROENTEROLOGY, 1995, 109 (03) :723-734
[8]  
DEKKER J, 1990, J BIOL CHEM, V265, P18116
[9]  
Forstner Janet F., 1994, P1255
[10]  
GENDLER SJ, 1990, J BIOL CHEM, V265, P15286