Reparable sonoporation generated by microstreaming

被引：123

作者：

Wu, JR ^{[1
]}

Ross, JP

Chiu, JF

机构：

[1] Univ Vermont, Dept Phys, Burlington, VT 05405 USA

[2] St Michaels Coll, Dept Chem & Phys, Colchester, VT 05439 USA

[3] Univ Vermont, Dept Biochem, Burlington, VT 05405 USA

来源：

JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA | 2002年 / 111卷 / 03期

关键词：

D O I：

10.1121/1.1420389

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

Reparable sonoporation was observed in Jurkat lymphocytes in suspension exposed to a vibrating Mason horn tuned to 21.4 KHz. The diameter of the horn tip was 400 mum and its transverse displacement amplitude was 7.8 mum. It was found that the shear stress associated with microstreaming surrounding the Mason-horn tip was the primary reason for the cell reparable sonoporation. The threshold shear stress was determined to be 12 +/- 4 Pa for exposure time up to 7 min. It was also found that the shorter the exposure time, the greater the threshold. (D 2002 Acoustical Society of America.

引用

页码：1460 / 1464

页数：5

共 14 条

[1]

[Anonymous], ULTRASOUND BIOL EFFE

[2] Transfection of a reporter plasmid into cultured cells by sonoporation in vitro [J].