HIV-specific cytotoxic T lymphocyte precursors exist in a CD28-CD8+T cell subset and increase with loss of CD4 T cells

被引:24
作者
Lewis, DE [1 ]
Yang, LX [1 ]
Luo, W [1 ]
Wang, XP [1 ]
Rodgers, JR [1 ]
机构
[1] Baylor Coll Med, Dept Microbiol & Immunol, Immunol Sect, Houston, TX 77030 USA
关键词
HIV; cytotoxic T lymphocyte; CD28; flow cytometry;
D O I
10.1097/00002030-199906180-00005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Objectives: To determine whether the CD28-CD8+ T cells that develop during HIV infection contain HIV-specific cytotoxic precursor cells. Design: CD8 subpopulations from six asymptomatic HIV-positive adults, with varying degrees of CD4 T cell loss, were sorted by flaw cytometry and HIV-specific precursor cytotoxic T lymphocyte frequencies were measured. Three populations of CD8 T cells were tested: CD28+CD57- T cells, CD28-CD57+ T cells (thought to be memory cells) and CD28-CD57- T cells (function unknown). Methods: Sorted CD8 subsets were stimulated with antigen presenting cells expressing HIV-1 Gag/Pol molecules. Cytotoxic T cell assays on Gag/Pol expressing Cr-51-labeled Epstein-Barr virus transformed autologous B cells lines or control targets were performed after 2 weeks. Specific lysis and precursor frequencies were calculated. Results: Both CD28 positive and CD28-CD57+ populations contained appreciable numbers of precursors (9-1720 per 10(6) CD8+ T cells). However, the CD28-CD57-population had fewer precursors in five out of six people studied. More CD28 positive HIV-specific cytotoxic T lymphocyte precursors were found in patients with CD4:CD8 ratios > 1, whereas more CD28-CD57+ precursors were found in patients whose CD 1 : CD8 ratios were < 1 (r(2), 0.68). Conclusions: Memory HIV-specific precursor cytotoxic T lymphocytes are found in both CD28 positive and CD28-CD8+ cells, however, a CD28-CD57-subpopulation had fewer. Because CD28-CD57+ cells are antigen-driven with limited diversity, the loss of CD28 on CD8 T cells during disease progression may reduce the response to new HIV mutations; this requires further testing. (C) 1999 Lippincott Williams & Wilkins.
引用
收藏
页码:1029 / 1033
页数:5
相关论文
共 33 条
[1]   Phenotypic analysis of antigen-specific T lymphocytes [J].
Altman, JD ;
Moss, PAH ;
Goulder, PJR ;
Barouch, DH ;
McHeyzerWilliams, MG ;
Bell, JI ;
McMichael, AJ ;
Davis, MM .
SCIENCE, 1996, 274 (5284) :94-96
[2]  
AZUMA M, 1993, J IMMUNOL, V150, P1147
[3]   Longitudinal study of HIV-specific cytotoxic lymphocytes in HIV type 1-infected patients: Relative balance between host immune response and the spread of HIV type 1 infection [J].
Bariou, C ;
Genetet, N ;
Ruffault, A ;
Michelet, C ;
Cartier, F ;
Genetet, B .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1997, 13 (15) :1301-1312
[4]   VIRUS-SPECIFIC CD8+ CYTOTOXIC T-LYMPHOCYTE ACTIVITY ASSOCIATED WITH CONTROL OF VIREMIA IN PRIMARY HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION [J].
BORROW, P ;
LEWICKI, H ;
HAHN, BH ;
SHAW, GM ;
OLDSTONE, MBA .
JOURNAL OF VIROLOGY, 1994, 68 (09) :6103-6110
[5]  
Boudet F, 1996, J IMMUNOL, V156, P2282
[6]   EXPRESSION OF COSTIMULATORY MOLECULE CD28 ON T-CELLS IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION - FUNCTIONAL AND CLINICAL CORRELATIONS [J].
BRINCHMANN, JE ;
DOBLOUG, JH ;
HEGER, BH ;
HAAHEIM, LL ;
SANNES, M ;
EGELAND, T .
JOURNAL OF INFECTIOUS DISEASES, 1994, 169 (04) :730-738
[7]   QUANTITATIVE-ANALYSIS OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1)-SPECIFIC CYTOTOXIC LYMPHOCYTE-T (CTL) RESPONSE AT DIFFERENT STAGES OF HIV-1 INFECTION - DIFFERENTIAL CTL RESPONSES TO HIV-1 AND EPSTEIN-BARR-VIRUS IN LATE DISEASE [J].
CARMICHAEL, A ;
JIN, X ;
SISSONS, P ;
BORYSIEWICZ, L .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (02) :249-256
[8]  
CHOREMIPAPADOPOULOU H, 1994, J ACQ IMMUN DEF SYND, V7, P245
[9]   VIRUS BURDEN IN LONG-TERM SURVIVORS OF HUMAN-IMMUNODEFICIENCY-VIRUS (HIV) INFECTION IS A DETERMINANT OF ANTI-HIV CD8(+) LYMPHOCYTE ACTIVITY [J].
FERBAS, J ;
KAPLAN, AH ;
HAUSNER, MA ;
HULTIN, LE ;
MATUD, JL ;
LIU, ZY ;
PANICALI, DL ;
NERNGHO, H ;
DETELS, R ;
GIORGI, JV .
JOURNAL OF INFECTIOUS DISEASES, 1995, 172 (02) :329-339
[10]   Predominant involvement of CD8(+)CD28(-) lymphocytes in human immunodeficiency virus-specific cytotoxic activity [J].
Fiorentino, S ;
Dalod, M ;
Olive, D ;
Guillet, JG ;
Gomard, E .
JOURNAL OF VIROLOGY, 1996, 70 (03) :2022-2026