Peroxidase-incorporated hydrophilic polythiophene electrode for the determination of hydrogen peroxide in acetonitrile

Peroxidase-incorporated conducting polymer electrodes are examined as hydrogen peroxide sensors in acetonitrile. An aqueous solution containing horseradish peroxidase (HRP) and chemically polymerized poly(3-(3'-thienyl)propanesulfonic acid) (PTS) was cast on SnO2-coated glass plates to obtain HRP/PTS electrodes. The electrode exhibits cathodic current responses to hydrogen peroxide (greater than or equal to 1 mu M) at + 100 to + 700 mV vs. Ag/Ag+ in acetonitrile containing 4% water and 0.1 M supporting salt (NaClO4 or tetra-n-butylammonium perchlorate).