Electrophoretic separation and characterisation of gliadin fractions from isolates and nanoparticulate drug delivery systems

Gliadin is a group of polymorphic proteins that are commonly used for the preparation of many pharmaceutical and cosmetic products. The aim of this study was to develop an analytical HPCE procedure fbr the identification and quantification of gliadin samples. For this purpose, the HPCE buffer composition were optimised to improve the resolution of separations. Therefore, several buffer modifiers were tested for use with 50 mM phosphate buffer in HPCE separations. Twenty percent acetonitrile and 0.05 % hydroxypropylmethylcellulose provided optimal resolution while maintaining excellent reproducibility. In conclusion, this method can be successfully applied to the elucidation of the different gliadin fractions in protein isolates and nanoparticulate dosage forms.