Telomeric proteins regulate episomal maintenance of Epstein-Barr virus origin of plasmid replication

被引：105

作者：

Deng, Z ^{[1
]}

Lezina, L ^{[1
]}

Chen, CJ ^{[1
]}

Shtivelband, S ^{[1
]}

So, W ^{[1
]}

Lieberman, PM ^{[1
]}

机构：

[1] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA

来源：

MOLECULAR CELL | 2002年 / 9卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S1097-2765(02)00476-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Episomal maintenance and DNA replication of EBV origin of plasmid replication (OriP) plasmid maintenance is mediated by the viral encoded origin binding protein, EBNA1, and unknown cellular factors. We found that telomeric repeat binding factor 2 (TRF2), TRF2-interacting protein hRap1, and the telomereassociated poly(ADP-ribose) polymerase (Tankyrase) bound to the dyad symmetry (DS) element of OriP in an EBNA1-dependent manner. TRF2 bound cooperatively with EBNA1 to the three nonamer sites (TTAGGGTTA), which resemble telomeric repeats. Mutagenesis of the nonamers reduced plasmid maintenance function and increased plasmid sensitivity to genotoxic stress. DS affinity-purified proteins possessed poly(ADP-ribose) polymerase (PARP) activity, and EBNA1 was subject to NAD-dependent posttranslational modification in vitro. OriP plasmid maintenance was sensitive to changes in cellular PARP/Tankyrase activity. These findings imply that telomere-associated proteins regulate OriP plasmid maintenance by PAR-dependent modifications.

引用

页码：493 / 503

页数：11

共 65 条

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