Enzyme dynamics and activity:: Time-scale dependence of dynamical transitions in glutamate dehydrogenase solution

被引:77
作者
Daniel, RM
Finney, JL
Réat, V
Dunn, R
Ferrand, M
Smith, JC
机构
[1] Univ Waikato, Dept Biol Sci, Hamilton, New Zealand
[2] Univ London Univ Coll, Dept Phys & Astron, London WC1E 6BT, England
[3] Inst Max Von Laue Paul Langevin, F-38042 Grenoble 9, France
[4] CEA Saclay, DBCM, SBPM, F-91191 Gif Sur Yvette, France
[5] Heidelberg Univ, IWR, Lehrstuhl Biocomp, D-69120 Heidelberg, Germany
关键词
D O I
10.1016/S0006-3495(99)77058-X
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We have examined the temperature dependence of motions in a cryosolution of the enzyme glutamate dehydrogenase (GDH) and compared these with activity. Dynamic neutron scattering was performed with two instruments of different energy resolution, permitting the separate determination of the average dynamical mean square displacements on the sub-similar to 100 ps and sub-similar to 5 ns time scales. The results demonstrate a marked dependence on the time scale of the temperature profile of the mean square displacement. The lowest temperature at which anharmonic motion is observed is heavily dependent on the time window of the instrument used to observe the dynamics. Several dynamical transitions (inflexions of the mean squared displacement) are observed in the slower dynamics. Comparison with the temperature profile of the activity of the enzyme in the same solvent reveals dynamical transitions that have no effect on GDH function.
引用
收藏
页码:2184 / 2190
页数:7
相关论文
共 31 条
[1]  
[Anonymous], [No title captured]
[2]  
BAKER PJ, 1997, BIOCHEMISTRY-US, V36, P16105
[3]  
Bee M., 1988, QUASIELASTIC NEUTRON
[4]   Harmonic behavior of trehalose-coated carbon-monoxy-myoglobin at high temperature [J].
Cordone, L ;
Ferrand, M ;
Vitrano, E ;
Zaccai, G .
BIOPHYSICAL JOURNAL, 1999, 76 (02) :1043-1047
[5]   TEMPERATURE-DEPENDENCE OF THE LOW-FREQUENCY DYNAMICS OF MYOGLOBIN - MEASUREMENT OF THE VIBRATIONAL FREQUENCY-DISTRIBUTION BY INELASTIC NEUTRON-SCATTERING [J].
CUSACK, S ;
DOSTER, W .
BIOPHYSICAL JOURNAL, 1990, 58 (01) :243-251
[6]   Enzyme activity below the dynamical transition at 220 K [J].
Daniel, RM ;
Smith, JC ;
Ferrand, M ;
Héry, S ;
Dunn, R ;
Finney, JL .
BIOPHYSICAL JOURNAL, 1998, 75 (05) :2504-2507
[7]  
DANIEL RM, 1997, BIOCHEM J, V317, P1
[8]   PROTEIN DYNAMICS - VIBRATIONAL COUPLING, SPECTRAL BROADENING MECHANISMS, AND ANHARMONICITY EFFECTS IN CARBONMONOXY HEME-PROTEINS STUDIED BY THE TEMPERATURE-DEPENDENCE OF THE SORET BAND LINESHAPE [J].
DIPACE, A ;
CUPANE, A ;
LEONE, M ;
VITRANO, E ;
CORDONE, L .
BIOPHYSICAL JOURNAL, 1992, 63 (02) :475-484
[9]   DYNAMICAL TRANSITION OF MYOGLOBIN REVEALED BY INELASTIC NEUTRON-SCATTERING [J].
DOSTER, W ;
CUSACK, S ;
PETRY, W .
NATURE, 1989, 337 (6209) :754-756
[10]   THERMAL MOTIONS AND FUNCTION OF BACTERIORHODOPSIN IN PURPLE MEMBRANES - EFFECTS OF TEMPERATURE AND HYDRATION STUDIED BY NEUTRON-SCATTERING [J].
FERRAND, M ;
DIANOUX, AJ ;
PETRY, W ;
ZACCAI, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9668-9672