Comparative Bacterial Proteomics: Analysis of the Core Genome Concept

被引：52

作者：

Callister, Stephen J. ^{[1
]}

McCue, Lee Ann ^{[2
]}

Turse, Joshua E. ^{[1
]}

Monroe, Matthew E. ^{[1
]}

Auberry, Kenneth J. ^{[3
]}

Smith, Richard D. ^{[1
]}

Adkins, Joshua N. ^{[1
]}

Lipton, Mary S. ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA

[2] Pacific NW Natl Lab, Comp Sci & Math Div, Richland, WA USA

[3] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA USA

来源：

PLOS ONE | 2008年 / 3卷 / 02期

关键词：

D O I：

10.1371/journal.pone.0001542

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

While comparative bacterial genomic studies commonly predict a set of genes indicative of common ancestry, experimental validation of the existence of this core genome requires extensive measurement and is typically not undertaken. Enabled by an extensive proteome database developed over six years, we have experimentally verified the expression of proteins predicted from genomic ortholog comparisons among 17 environmental and pathogenic bacteria. More exclusive relationships were observed among the expressed protein content of phenotypically related bacteria, which is indicative of the specific lifestyles associated with these organisms. Although genomic studies can establish relative orthologous relationships among a set of bacteria and propose a set of ancestral genes, our proteomics study establishes expressed lifestyle differences among conserved genes and proposes a set of expressed ancestral traits.

引用

页数：8

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