The rpoH gene encoding sigma(32) homolog of Vibrio cholerae

被引：19

作者：

Sahu, GK ^{[1
]}

Chowdhury, R ^{[1
]}

Das, J ^{[1
]}

机构：

[1] INDIAN INST CHEM BIOL, DIV BIOPHYS, Kolkata 700032, W BENGAL, INDIA

来源：

GENE | 1997年 / 189卷 / 02期

关键词：

heat shock response; sigma factor; rpoH box; mRNA secondary structure; bidirectional transcription terminator; udhA gene;

D O I：

10.1016/S0378-1119(96)00849-9

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Vibrio cholerae rpoH gene coding for the heat-shock sigma factor, sigma(32), has been cloned and shown to functionally complement Escherichia coli rpoH mutants. The nt sequence of the gene has been determined and the deduced aa sequence is more than 80% homologous to the E. coli rpoH gene product. Downstream of the V. cholerae rpoH gene, an unidentified dehydrogenase gene (udhA) is present on the opposite strand facing rpoH. The predicted secondary structure of the 5'-proximal region of V. cholerae rpoH mRNA is apparently different from the conserved secondary structures of the rpoH mRNA reported for several bacterial species. The 'RpoH box', a stretch of 9 aa (QRKLFFNLR) unique to sigma(32) factors, and the 'downstream box' sequence complementary to a part of the 16S rRNA, have been detected. (C) 1997 Elsevier Science B.V.

引用

页码：203 / 207

页数：5

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