Molecular characterization of a ribonucleotide reductase (nrdF) gene fragment of Mycoplasma hyopneumoniae and assessment of the recombinant product as an experimental vaccine for enzootic pneumonia

被引：22

作者：

Fagan, PK

Djordjevic, SP

Eamens, GJ

Chin, J

Walker, MJ

机构：

[1] ELIZABETH MACARTHUR AGR INST, MICROBIOL & IMMUNOL SECT, CAMDEN, NSW 2570, AUSTRALIA

[2] UNIV WOLLONGONG, DEPT BIOL SCI, WOLLONGONG, NSW 2500, AUSTRALIA

来源：

INFECTION AND IMMUNITY | 1996年 / 64卷 / 03期

关键词：

D O I：

10.1128/IAI.64.3.1060-1064.1996

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A Mycoplasma hyopneumoniae clone bank was screened with hyperimmune pig serum. One clone exhibited sequence homology to the prokaryotic R2 subunit of ribonucleotide reductase and was expressed as an 11-kDa protein fused to beta-galactosidase. The vaccine potential of the fusion protein was assessed in pig trials. Following experimental challenge with a virulent isolate of M. hyopneumoniae, gross lung pathology (mean Goodwin lung score) of vaccinated animals, irrespective of adjuvant treatment, was significantly reduced compared with that of control unvaccinated pigs (P < 0.05).

引用

页码：1060 / 1064

页数：5

共 40 条

[1]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[2]

BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5

[3]

CIPRIAN A, 1988, CAN J VET RES, V52, P434

[4] ALGAMMULIN, A NEW VACCINE ADJUVANT COMPRISING GAMMA INULIN PARTICLES CONTAINING ALUM - PREPARATION AND INVITRO PROPERTIES [J].