Direct Identification of Bifidobacteria from Probiotic Supplements

被引:4
作者
Bunesova, Vera [1 ]
Vlkova, Eva [1 ]
Rada, Vojtech [1 ]
Hovorkova, Petra [1 ]
Musilova, Sarka [1 ]
Kmet, Vladimir [2 ]
机构
[1] Czech Univ Life Sci Prague, Fac Agrobiol Food & Nat Resources, Dept Microbiol Nutr & Dietet, Kamycka 129, Prague 16521 6, Czech Republic
[2] Slovak Acad Sci, Inst Anim Physiol, Kosice, Slovakia
关键词
Bifidobacterium; species-specific identification; DGGE ANALYSIS; PRODUCTS; ENUMERATION; LACTIS; FOOD;
D O I
10.17221/291/2013-CJFS
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The DNA of 14 probiotic supplements was isolated directly from various products without previous cultivation. The bifidobacteria composition declared by the manufacturer was determined by species-specific PCR. Such species found were in accordance with those listed on the products. This approach can be performed in less than 5 h and is applicable to other genera of probiotic bacteria. Bifidobacteria were also detected by culture-dependent analysis and MALDI-TOF MS. These methods drew the same results; however, they are more expensive and time consuming. So, we propose direct identification of bifidobacteria for routine quality control inspections of species composition in probiotic supplements.
引用
收藏
页码:132 / 136
页数:5
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