Integron-located oxa-32 gene cassette encoding an extended-spectrum variant of OXA-2 β-lactamase from Pseudomonas aeruginosa

被引：31

作者：

Poirel, L

Gerome, P

De Champs, C

Stephanazzi, J

Naas, T

Nordmann, P

机构：

[1] Hop Bicetre, Serv Bacteriol Virol, Fac Med Paris Sud, Assistance Publ Hop Paris, F-94275 Le Kremlin Bicetre, France

[2] Hop Instruct Armees Percy, Serv Biol, F-92140 Clamart, France

[3] Hop Instruct Armees Percy, Ctr Traitement Brules, F-92140 Clamart, France

[4] Univ Auvergne, Fac Med, Bacteriol Lab, F-63001 Clermont Ferrand, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2002年 / 46卷 / 02期

关键词：

D O I：

10.1128/AAC.46.2.566-569.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pseudomonas aeruginosa clinical isolate CY-1, which was resistant to ceftazidime, harbored a conjugative ca. 250-kb plasmid that contained a class 1 integron with two gene cassettes encoding OXA-32, an OXA-2- type beta-lactamase, and the aminoglycoside acetyltransferase AAC(6')Ib(9). OXA-32 differed from OXA-2 by an Leu169Ile amino acid substitution (class D numbering). Site-directed mutagenesis established that Ile169 is responsible for resistance to ceftazidime but not to cefotaxime.

引用

页码：566 / 569

页数：4

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