Cytosolic dehydroascorbate reductase is important for ozone tolerance in Arabidopsis thaliana

被引：87

作者：

Yoshida, S

Tamaoki, M ^{[1
]}

Shikano, T

Nakajima, N

Ogawa, D

Ioki, M

Aono, M

Kubo, A

Kamada, H

Inoue, Y

Saji, H

机构：

[1] Natl Inst Environm Studies, Biodivers Conservat Res Project, Tsukuba, Ibaraki 3058506, Japan

[2] Univ Tsukuba, Masters Program Biosyst Studies, Tsukuba, Ibaraki 3058577, Japan

[3] Univ Tsukuba, Grad Sch Life & Environm Sci, Tsukuba, Ibaraki 3058577, Japan

[4] Natl Inst Environm Studies, Div Environm Biol, Tsukuba, Ibaraki 3058506, Japan

[5] Tokyo Univ Sci, Chiba 2788510, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2006年 / 47卷 / 02期

关键词：

Arabidopsis thaliana; apoplast; ascorbate; dehydroascorbate reductase; oxidative stress; ozone;

D O I：

10.1093/pcp/pci246

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Dehydroascorbate reductase (DHAR) is a key component of the ascorbate recycling system. Three functional DHAR genes are encoded in the Arabidopsis genome. Ozone exposure increased the expression of the cytosolic DHAR (cytDHAR) gene alone. We characterized an Arabidopsis mutant with a deficient cytDHAR. The mutant completely lacked cytDHAR activity and was highly ozone sensitive. The amounts of total ascorbate and glutathione were similar in both lines, but the amount of apoplastic ascorbate in the mutant was 61.5% lower. These results indicate that the apoplastic ascorbate, which is generated through the reduction of DHA by cytDHAR, is important for ozone tolerance.

引用

页码：304 / 308

页数：5

共 22 条

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