Cannabinoid receptor CB1-like and glutamic acid decarboxylase-like immunoreactivities in the brain of Xenopus laevis

被引:23
作者
Cesa, R
Mackie, K
Beltramo, M
Franzoni, MF
机构
[1] Univ Turin, Dipartimento Biol Anim Uomo, I-10123 Turin, Italy
[2] Univ Washington, Dept Anesthesiol, Seattle, WA 98195 USA
[3] Univ Washington, Dept Physiol & Biophys, Seattle, WA 98195 USA
[4] Schering Plough Res Inst, Milan, Italy
关键词
cannabinoid receptor CB1; glutamic acid decarboxylase; confocal microscopy; Xenopus laevis (Anura);
D O I
10.1007/s004410100461
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Investigation of the cannabinoid system in a vertebrate group phylogenetically distant from mammals might improve understanding of its physiological role. Thus, in the present study, the distribution of the cannabinoid CB 1 receptor has been investigated in the brain of Xenopus laevis (anuran amphibians) by immunohistochemistry, using both light and confocal laser-scanning microscopy. Immunostained neuronal perikarya and terminals were found in the olfactory bulb, dorsal and medial pallium, striatum, and amygdala. Varicosities and nerve terminals containing CB1-like immunoreactivity were also seen in the thalamus and hypothalamus. A number of stained cells were observed in the pars distalis of the pituitary gland. Positive nerve fibers were distributed throughout mesencephalic tegmentum, and in the cerebellum immunolabeling was observed in some Purkinje and possibly Golgi cells. The confocal microscopic analysis of CB1-like and glutamic acid decarboxylase-like immunoreactivities in both the medial pallium of the telencephalon and the olfactory bulbs showed a wide codistribution of the two markers. The present results indicate that distribution of CB I is conserved in the course of phylogeny. Furthermore, the close relationship between CBI-like and glutamic acid decarboxylase-like immunolabelings point toward the existence of a functional link between cannabinergic and GABAergic innervations also in amphibian brain.
引用
收藏
页码:391 / 398
页数:8
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