Loss of heterozygosity of 14q32 in colorectal carcinoma

被引：70

作者：

Bando, T

Kato, Y

Ihara, Y

Yamagishi, F

Tsukada, K

Isobe, M

机构：

[1] Toyama Med & Pharmaceut Univ, Sch Med, Dept Surg 2, Toyama 9300194, Japan

[2] Toyama Univ, Dept Mat & Biosyst Engn, Fac Engn, Dept Cell & Mol Biol, Toyama 930, Japan

来源：

CANCER GENETICS AND CYTOGENETICS | 1999年 / 111卷 / 02期

关键词：

D O I：

10.1016/S0165-4608(98)00242-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Previous allelotyping studies on colorectal carcinoma suggest that loss of heterozygosity (LOH) on chromosome 14q may be a common genetic alteration in this tumor type. The purpose of this study was to determine a precise frequency of LOH at 14q32 region in colorectal carcinomas and to define a minimal region of LOH. LOH at 14q32 in 66 primary colorectal carcinomas were analyzed by polymerase chain reaction-based deletion mapping using six highly polymorphic microsatellites. The average rate of informative data was 68.2% in all cases of colorectal carcinomas analyzed. The average rate of LOH at 14q32 was 44.1%, with the highest frequency of 50.0% at D14S267 locus in the informative cases. The minimal region of LOH was defined by markers D14S65 and D14S250 at 14q32. The present data provide useful information for the isolation of tumor suppressor genes from this region. (C) Elsevier Science Inc., 1999. All rights reserved.

引用

页码：161 / 165

页数：5

共 23 条

[1]

DIAGONAL CUBIC EQUATIONS .2. [J].