Muscarinic acetylcholine receptors in the hippocampus, neocortex and amygdala: A review of immunocytochemical localization in relation to learning and memory

Immunocytochemical mapping studies employing the extensively used monoclonal antimuscarinic acetylcholine: receptor (mAChR) antibody M35 are reviewed;sd. We focus on three neuronal muscarinic cholinoceptive substrates. which are target regions of the cholinergic basal forebrain system intimately involved in cognitive functions: the hippocampus: neocortex: and amygdala. Thr distribution and neurochemistry of mAChR-immunoreactive cells as wall as behaviorally induced alterations in mAChR-immunoreactivity; (ir) are described in detail. M35(+) neurons are viewed as cells actively engaged in neuronal functions in which the cholinergic system is typically involved. Phosphorylation and subsequent internalization of muscarinic receptors determine the immunocytochemical outcome, and hence M35 as a tool to visualize muscarinic receptors is less suitable for detection of the entire pool of mAChRs in the central nervous system (CNS). Instead, M35 is sensitive to and capable of detecting alterations in the physiological condition of muscarinic receptors. Therefore. M35 is an excellent tool to, localize alterations in cellular cholinoceptivity in the CNS. M35-ir is not only deter mined by acetylcholine (ACh), but by any substance that changes the phosphorylation/internalization state of the mAChR. An important consequence of this proposition is that other neurotransmitters than ACh (especially; glutamate) can regulate M35-ir and the cholinoceptive state of a neuron. and hence the functional properties of a neuron. One of the primary objectives of this review is to provide a synthesis of our data and literature data on mAChR-ir. We propose a hypothesis: for the role of muscarinic receptors in learning and memory in terms of modulation between learning and recall states of brain areas at the postsynaptic level as studied by way of immunocytochemistry employing the monoclonal antibody M35. (C) 1999 Elsevier Science Ltd. All rights reserved.