A new method to induce multi-drug resistance to carboplatin in a mouse model of human tongue squamous cell carcinoma

被引：9

作者：

Feng, G. ^{[1
]}

Wang, D. -Z. ^{[2
]}

Chen, H. -Q. ^{[3
]}

Hu, J. ^{[2
]}

He, J. ^{[1
]}

机构：

[1] Sichuan Univ, State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, W China Stomatol Coll, Dept Oral & Maxillofacial Surg, Chengdu 610041, Sichuan, Peoples R China

[3] Sichuan Univ, W China Ctr Med Sci, Inst Biomed Engn, Chengdu 610041, Sichuan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY | 2008年 / 37卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Tca8113 cell line; human tongue squamous cell carcinoma; multi-drug resistance; carboplatin;

D O I：

10.1016/j.ijom.2008.06.014

中图分类号：

R78 [口腔科学];

学科分类号：

1003 [口腔医学];

摘要：

Multi-drug resistance (MDR) in human head and neck squamous cell carcinoma (HNSCC) constitutes a major obstacle to the effectiveness of chemotherapy. In previous studies, MDR was mainly induced in vitro. The authors report a novel in vivo method of inducing MDR in nude mice with xenotransplanted Tca8113 cells. Carboplatin, a chemotherapeutic agent used to treat HNSCC, was injected around the tumors for 10 weeks. A subsequent cell survival assay of dissociated tumor cells suggested that MDR had been induced successfully. Immunocytochemistry, reverse transcription polymerase chain reaction and Western blot analysis showed that the expression levels of MDR-related proteins, including topoisomerase II, MRP and glutathione transferase, were elevated in the induction group. The authors conclude that in vivo induction of MDR provides a useful method for establishing animal models of MDR.

引用

页码：1141 / 1147

页数：7

共 22 条

[1]

Determination of the DNA-binding characteristics of ethidium bromide, proflavine, and cisplatin by flow injection analysis:: Usefulness in studies on antitumor drugs [J].