Genotyping by "cold single-strand conformation polymorphism" of the UGT1A1 promoter polymorphism in Mexican mestizos

Since no data have previously been reported concerning both the (TA)(n) polymorphism at the promoter of the UGT1A1 gene in the Mexican population and the use of single-strand conformation polymorphism (SSCP) for the detection of such polymorphism, genotyping by SSCP in 375 G-6-PD normal (Group A) and 81 G-6-PD-deficient (Group B) mestizos belonging to 14 states was carried out. Allele frequencies for (TA)(6) and (TA)(7) repeats were 0.654 and 0.334, respectively, in Group A and 0.685 and 0.315 in Group B: in the former group, the (TA)(5) allele was also observed with a frequency of 0.012. The frequencies of the genotype (TA)(7)/(TA)(7) were 10.1% (Group A) and 8.6% (Group B). The (TA)(7)/(TA)(8) genotype was also observed in a patient with unconjugated hyperbilirubinemia. Due to the importance of its potential medical implications, the observed high frequency (10%) of the (TA)(7)/(TA)(7) genotype is stressed. Genotyping by SSCP of the (TA)(n) polymorphism is an adequate methodological option. (C) 2002 Elsevier Science (USA).