Cytotoxic T lymphocytes to Ebola Zaire virus are induced in mice by immunization with liposomes containing lipid A

被引:40
作者
Rao, M [1 ]
Matyas, GR
Grieder, F
Anderson, K
Jahrling, PB
Alving, CR
机构
[1] Walter Reed Army Inst Res, Dept Membrane Biochem, Washington, DC 20307 USA
[2] Uniformed Serv Univ Hlth Sci, Dept Immunol & Microbiol, Bethesda, MD 20814 USA
[3] USA, Med Res Inst Infect Dis, Div Virol, Frederick, MD 21702 USA
关键词
liposomes; Ebola virus; CTL;
D O I
10.1016/S0264-410X(99)00170-X
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
An eight amino acid sequence (TELRTFSI) present in the carboxy terminal end (aa 577-584) of membrane-anchored GP, the major structural protein of Ebola virus, was identified as an H-2(k)-specific murine cytotoxic T cell epitope, Cytotoxic T lymphocytes (CTLs) to this epitope were induced by immunizing B10.BR mice intravenously with either irradiated Ebola virus or with irradiated Ebola virus encapsulated in liposomes containing lipid A. The CTL response induced by irradiated Ebola virus could not be sustained after the second round of in vitro stimulation of immune splenocytes with the peptide, unless the irradiated virus was encapsulated in liposomes containing lipid A. The identification of an Ebola GP-specific CTL epitope and the requirement of liposomal lipid A for CTL memory recall responses could prove to be a promising approach for developing a vaccine against Ebola virus infection. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:2991 / 2998
页数:8
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