Overexpression of poly(A)-binding protein down-regulates the translation or the abundance of its own mRNA

被引:33
作者
Hornstein, E [1 ]
Harel, H [1 ]
Levy, G [1 ]
Meyuhas, O [1 ]
机构
[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Dept Biochem, IL-91120 Jerusalem, Israel
基金
以色列科学基金会;
关键词
translational regulation; poly(A)-binding protein; polysome; autoregulation;
D O I
10.1016/S0014-5793(99)01039-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Poly(A)-binding protein (PABP) mRNA is subject to autoregulation through a 61 nucleotides long A-rich sequence in its 5' untranslated region (UTR), Here, we show that this mode of regulation is exerted in a cell type-specific manner, Thus, overexpression of PABP in mouse NIH 3T3 fibroblasts represses the translation of the respective endogenous mRNA or that of a chimeric mRNA containing just the 5' UTR of PABP mRNA, In contrast, ectopic expression of PABP in human embryonic kidney 293 cells down-regulates the abundance of the endogenous PABP mRNA, rather than affecting its translational efficiency. Transfection experiments with chimeric constructs suggest that the lack of translational autoregulation of endogenous PABP mRNA in these cells appears to reflect the presence of an overriding regulatory element outside the A-rich region. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:209 / 213
页数:5
相关论文
共 46 条
[1]   Yeast Pab1 interacts with Rna15 and participates in the control of the poly(A) tail length in vitro [J].
Amrani, N ;
Minet, M ;
LeGouar, M ;
Lacroute, F ;
Wyers, F .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (07) :3694-3701
[2]   The 5' terminal oligopyrimidine-tract confers translational control on TOP mRNAs in a cell-type and sequence context-dependent manner [J].
Avni, D ;
Biberman, Y ;
Meyuhas, O .
NUCLEIC ACIDS RESEARCH, 1997, 25 (05) :995-1001
[3]   VERTEBRATE MESSENGER-RNAS WITH A 5'-TERMINAL PYRIMIDINE TRACT ARE CANDIDATES FOR TRANSLATIONAL REPRESSION IN QUIESCENT CELLS - CHARACTERIZATION OF THE TRANSLATIONAL CIS-REGULATORY ELEMENT [J].
AVNI, D ;
SHAMA, S ;
LORENI, F ;
MEYUHAS, O .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (06) :3822-3833
[4]   Translational control of poly(A)-binding protein expression [J].
Bag, J ;
Wu, J .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 237 (01) :143-152
[5]   Autoregulation of protein phosphatase type 2A expression [J].
Baharians, Z ;
Schönthal, AH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (30) :19019-19024
[6]   EXPRESSION OF 2 XENOPUS-LAEVIS RIBOSOMAL-PROTEIN GENES IN INJECTED FROG OOCYTES - A SPECIFIC SPLICING BLOCK INTERFERES WITH THE L1 RNA MATURATION [J].
BOZZONI, I ;
FRAGAPANE, P ;
ANNESI, F ;
PIERANDREIAMALDI, P ;
AMALDI, F ;
BECCARI, E .
JOURNAL OF MOLECULAR BIOLOGY, 1984, 180 (04) :987-1005
[7]   Poly(A) tail length control in Saccharomyces cerevisiae occurs by message-specific deadenylation [J].
Brown, CE ;
Sachs, AB .
MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (11) :6548-6559
[8]   THE ACCUMULATION OF MATURE RNA FOR THE XENOPUS-LAEVIS RIBOSOMAL PROTEIN-L1 IS CONTROLLED AT THE LEVEL OF SPLICING AND TURNOVER OF THE PRECURSOR RNA [J].
CAFFARELLI, E ;
FRAGAPANE, P ;
GEHRING, C ;
BOZZONI, I .
EMBO JOURNAL, 1987, 6 (11) :3493-3498
[9]   HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].
CHEN, C ;
OKAYAMA, H .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752
[10]   IDENTIFICATION OF A THYMIDYLATE SYNTHASE RIBONUCLEOPROTEIN COMPLEX IN HUMAN COLON-CANCER CELLS [J].
CHU, E ;
VOELLER, DM ;
JONES, KL ;
TAKECHI, T ;
MALEY, GF ;
MALEY, F ;
SEGAL, S ;
ALLEGRA, CJ .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (01) :207-213