Genetic differences in cholesterol absorption in 129/Sv and C57BL/6 mice: effect on cholesterol responsiveness

被引:51
作者
Jolley, CD [1 ]
Dietschy, JM [1 ]
Turley, SD [1 ]
机构
[1] Univ Texas, SW Med Ctr, Dept Internal Med, Dallas, TX 75235 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 1999年 / 276卷 / 05期
关键词
cholesterol esterification; hepatic sterol synthesis; small intestine; fecal sterol excretion; cholesterol transport;
D O I
10.1152/ajpgi.1999.276.5.G1117
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
This study compared the cholesterolemic response of two strains of mice with genetically determined differences in cholesterol absorption. When fed a basal low-cholesterol diet, 129/Sv mice absorbed cholesterol twice as efficiently as did C57BL/6 mice (44% vs. 20%). Total lipid absorption, in contrast, averaged 80-82% in both strains. The higher level of cholesterol absorption in the 129/Sv animals was reflected in an adaptive reduction in hepatic and intestinal sterol synthesis. When fed lipid-enriched diets, the 129/Sv mice became significantly more hypercholesterolemic and had twofold higher hepatic cholesterol concentrations than did the C57BL/6 animals even though the conversion of cholesterol to bile acids was stimulated equally in both strains. The difference in cholesterol absorption between these mouse strains was not the result of physicochemical factors relating to the size and composition of the intestinal bile acid pool but more likely reflects an inherited difference in one or more of the biochemical steps that facilitate the translocation of sterol across the epithelial cell.
引用
收藏
页码:G1117 / G1124
页数:8
相关论文
共 44 条
[1]   Identification of a form of acyl-CoA:cholesterol acyltransferase specific to liver and intestine in nonhuman primates [J].
Anderson, RA ;
Joyce, C ;
Davis, M ;
Reagan, JW ;
Clark, M ;
Shelness, GS ;
Rudel, LL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (41) :26747-26754
[2]  
BHATTACHARYYA AK, 1980, J LIPID RES, V21, P518
[3]   Genetic variation in cholesterol absorption efficiency among inbred strains of mice [J].
Carter, CP ;
Howles, PN ;
Hui, DY .
JOURNAL OF NUTRITION, 1997, 127 (07) :1344-1348
[4]   ACAT-2, a second mammalian acyl-CoA:cholesterol acyltransferase -: Its cloning, expression, and characterization [J].
Cases, S ;
Novak, S ;
Zheng, YW ;
Myers, HM ;
Lear, SR ;
Sande, E ;
Welch', CB ;
Lusis, AJ ;
Spencer, TA ;
Krause, BR ;
Erickson, SK ;
Farese, RV .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (41) :26755-26764
[5]  
CLEEMAN JI, 1988, ARCH INTERN MED, V148, P36, DOI 10.1001/archinte.148.1.36
[6]   EFFECTS OF HYODEOXYCHOLIC ACID AND ALPHA-HYOCHOLIC ACID, 2 6-ALPHA-HYDROXYLATED BILE-ACIDS, ON CHOLESTEROL AND BILE-ACID METABOLISM IN THE HAMSTER [J].
COHENSOLAL, C ;
PARQUET, M ;
FEREZOU, J ;
SEROUGNE, C ;
LUTTON, C .
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1995, 1257 (02) :189-197
[7]   KNOCKOUT OF THE MOUSE APOLIPOPROTEIN-B GENE RESULTS IN EMBRYONIC LETHALITY IN HOMOZYGOTES AND PROTECTION AGAINST DIET-INDUCED HYPERCHOLESTEROLEMIA IN HETEROZYGOTES [J].
FARESE, RV ;
RULAND, SL ;
FLYNN, LM ;
STOKOWSKI, RP ;
YOUNG, SG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (05) :1774-1778
[8]  
Field FJ, 1998, J LIPID RES, V39, P1938
[9]   Identification of a receptor mediating absorption of dietary cholesterol in the intestine [J].
Hauser, H ;
Dyer, JH ;
Nandy, A ;
Vega, MA ;
Werder, M ;
Bieliauskaite, E ;
Weber, FE ;
Compassi, S ;
Gemperli, A ;
Boffelli, D ;
Wehrli, E ;
Schulthess, G ;
Phillips, MC .
BIOCHEMISTRY, 1998, 37 (51) :17843-17850
[10]  
Homan R, 1997, CURR PHARM DESIGN, V3, P29