Photophysics of a β-carboline based non-ionic probe in anionic and zwitterionic liposome membranes

被引：15

作者：

Das, Paramita ^{[1
]}

Sarkar, Deboleena ^{[1
]}

Chattopadhyay, Nitin ^{[1
]}

机构：

[1] Jadavpur Univ, Dept Chem, Kolkata 700032, India

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2008年 / 154卷 / 01期

关键词：

liposome; lipid-water interface; surface charge; micropolarity; fluorescence quenching; limiting anisotropy;

D O I：

10.1016/j.chemphyslip.2008.03.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interaction of a biologically active P-carboline based non-ionic probe, 3-acetyl-4-oxo-6,7-dihydro-12H indolo-[2,3-a] quinolizine (AODIQ), with the liposomal vesicles of dimyristoyl-L-alpha-phosphatidylcholine (DMPC) and dimyristoyl-L-alpha-phosphatidylglycerol (DMPG) has been demonstrated using steady-state and time-resolved fluorescence and fluorescence anisotropy techniques. Polarity sensitive intramolecular charge transfer of AODIQ shows a large hypsochromic shift along with an enhancement in the fluorescence quantum yield and fluorescence lifetime in the bilayer membranes compared to those in aqueous buffer solution. Polarity of the immediate vicinity of the probe in the lipid environments has been determined. The fluorometric, quenching and micropolarity determination studies reveal that the fluorophore penetrates deeper in the zwitterionic DMPC membrane compared to the anionic DMPG vesicle. Enhancement in the rotational relaxation time of AODIQ in liposomal membranes suggests that the fluorophore exists in motionally restricted environments. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：38 / 45

页数：8

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